Kai-Hong GuoPei-Hsuan ChenChieh LinChien-Fu ChenI-Ren LeeYi-Chun YehCHIEN-FU CHEN2018-09-102018-09-102018-03http://scholars.lib.ntu.edu.tw/handle/123456789/402162This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine. ? 2018 American Chemical Society.cueR; Cupriavidus metallidurans; paper device; smartphone-based diagnostic system; whole-cell biosensor[SDGs]SDG3gold; ion; luminescent agent; photoprotein; red fluorescent protein; chemistry; Cupriavidus; genetic engineering; genetic procedures; genetics; human; paper; procedures; urine; Biosensing Techniques; Cupriavidus; Genetic Engineering; Gold; Humans; Ions; Luminescent Agents; Luminescent Proteins; Paperjournal article10.1021/acssensors.7b009312-s2.0-85045875539WOS:000431165200002