2017-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684228摘要：嘉德麗雅蘭為西洋蘭花的代表，為更年性花卉，其切花對乙烯非常敏感，有花期過短之問題，本計畫期望利用生物技術，調控嘉德麗雅蘭對乙烯之敏感度，育成延長切花壽命的嘉德麗雅蘭。運用先前建立之嘉德麗雅蘭農桿菌媒介基因轉殖系統，已將含乙烯訊息傳導路徑負向調控因子 CTR1 (Constitutive Triple Response 1) 基因過量表現質體，以嘉德麗雅蘭類原球體及葉片為培殖體，進行基因轉殖，經抗生素篩選及再生所得到的擬轉殖株，經 GUS 活性組織化學染色分析、聚合&#37238;連鎖反應(polymerase chain reaction) 及南方氏雜交分析 (Southern blot analysis) 等分子驗證，已確認轉殖植株。本年度將進行轉殖株之外表性狀及花期調查，包括自然花期及乙烯處理花期，以瞭解過量表現 CTR1 對嘉德麗雅蘭花期之影響。<br> Abstract: Cattleya one of the favorite orchids in Westen world, is one of the climacteric flowers whose flowers are very sensitive to ethylene. To prolong the vase life of cut Cattleya flowers, manipulation of signal transduction in Cattleya flowers for reduction of ethylene sensitivity will be conducted in this project via genetic transformation. Using protocorm-like body (PLB) and leaf as explants, the construct containing gene encoding negative regulator CTR1 gene driven by CaMV 35S promoter has been transformed into Cattleya via Agrobacterium-mediated method. Transgenic Cattleya plants regenerated after selection by antibiotics had been confirmed by GUS histochemical staining, polymerase chain reaction, and Southern blot analsis. Phenotype and flower longevity of transgenic plants will be investigated this year.嘉德麗雅蘭乙烯訊息傳導路徑負向調控因子CTR1乙烯訊息傳導Cattleyaconstitutive triple response 1ethylene signal transduction