周宏農2006-07-262018-07-062006-07-262018-07-061999-07-31http://ntur.lib.ntu.edu.tw//handle/246246/20540建立本地有毒藻株的種源收集庫並 開始其毒素與毒性分析﹐提供台灣本土紅 潮及有害藻類活性物質的研究材料﹐在現 有91株藻種中包含有部份同種之有毒與無 毒藻株。由淡水水華微囊藻及分離培養之 藻株Microcystis aeruginosa MTN-2純化有 10種不同微囊藻毒﹐其中的MCYST-RA及 [D-Asp]MYCST-FR, -WR為新化合物。在 十二株微小亞歷山大藻Alexandrium tamarense中的九株發現含毒﹐且含毒藻株 各有不同之毒素組成﹐其中一株之含毒量 為目前所知同種中最高之一株。同時發現 其含毒藻株與其所分離毒素本身均會在虱 目魚之幼魚鰓部造成次級鰓板腫大及上皮 細胞的剝離﹐因而提高其耗氧率及對臨界 氧壓的需求﹐用以解釋養殖池中水華所導 致虱目魚的死亡﹐過去文獻尚無此相關之 發現。從原甲藻Prorocentrum lima, P.sp上 分離有下痢性毒素之okadiac acid, DTX-1 及其雙醇基醯化合物﹐包含一新雙醇基醯 之黑海綿酸﹐另外純化有原甲藻大環內酯 prorocentrolide, hydroxy-prorocentrolide及 一結晶之新化合物命名為 spiroprorocentrimine。To establish a toxic microalgal culture collection in Taiwan to support further studies on red tide and harmful algal toxins. Among 91 clones of different species, there are toxic and nontoxic strains of the same species. Ten microcystins have been isolated from field collection of blooming population of Microcystis aeruginosa and strain MTN-2 of the same species. Three of the toxins are known to be new in literature. Among 12 Alexandrium minutum isolates there were 9 found to be toxic, and their toxin profiles were different. One of the strain, AmTK6a was known as the most toxic one ever reported. It was also found toxic cells of A. minutum and the purified toxin showed toxicity to milkfish larvae by damaging fish gills and thus causing a high demand and consumption of oxygen in fish. From one Prorocentrum lima isolate, okadaic acid, its diol esters, and DTX-1 were isolated. One of the diol ester of OA was known as new compound. Spiroprorocentrimine, a new compound was isolated and crystalized from the extract of one unknown Prorocentrum species, in addition to prorocentrolide and hydroxyprorocentrolide.application/pdf53514 bytesapplication/pdfzh-TW國立臺灣大學漁業科學研究所原甲藻大環內酯微囊藻毒螺 接原甲藻大環內酯亞胺麻痺性貝毒利瑪原甲藻微小亞歷山大微囊藻prorocentrolidemicrocystinsspiroprorocentrimineparalytic shellfish toxinsProrocentrum limaAlexandrium minutumMicrocystis aeruginosajournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/20540/1/882312B002039.pdf