2008-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/686346摘要：臺灣杉 (Taiwania cryptomerioides Hayata) 為臺灣原生之重要經濟樹種，屬新生代第三紀孑遺植物，為世界級之珍貴「活化石」樹種。針對臺灣杉的抽出成分、生物活性、及其木材性質的研究，已累積相當多的成果。為進一步於分子層面了解臺灣杉生長發育之相關基因及其之間的調控，本計畫將延續我們對臺灣杉表現序列標籤基因庫中篩選所得與生長發育相關基因－Mago nashi及Y14－之初步研究，並配合酵母菌雙雜合系統、微陣列晶片、原位雜合與免疫組織化學等研究技術及策略，深入探討與Mago nashi及Y14具交互作用之蛋白質，及其於調控林木生長發育之機制。期能藉此有助於進一步了解Mago nashi、Y14及其相關基因於臺灣杉生長發育上扮演之角色。此外，此研究成果亦可望提供林木生理、林木改良、演化及保育之重要參考資料。<br> Abstract: Taiwania (Taiwania cryptomerioides Hayata) is one of the most economically important native tree species grown in Taiwan. Besides, it is the “living fossil” from the Tertiary period of the Cenozoic era. To consider in the wood utilization and bioactivity points of view, there are several research results of Taiwania published in international scientific journals. To further understand the genes of Taiwania related to growth, development and their regulation at the level of molecule, we will investigate the proteins which interact with Mago nashi and Y14 and their regular mechanisms on growth and development by the techniques of yeast two-hybrid system, microarray, in situ hybridization, immuno histochemistry based on the preliminary researches of Mago nashi and Y14 genes obtained from Taiwania expression sequence tags (ESTs) library. We hope it will facilitate to understand the roles of Mago nashi, Y14 and their related genes on the growth and development of Taiwania. Moreover, it will provide the useful information for the researches on tree physiology, tree improvement, evolution, and conservation in the future.臺灣杉生長發育酵母菌雙雜合系統微陣列晶片原位雜合免疫組織化學Taiwania cryptomerioidesgrowthdevelopmentyeast two-hybrid systemmicroarrayin situ hybridizationimmuno-histochemistry