2006-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662174摘要：臺灣杉 (Taiwania cryptomerioides Hayata) 是臺灣原產重要經濟樹種，與銀杏 (Ginkgo biloba)、世界爺 (Sequoiadendron ginanteum)及水杉 (Metasequoia glyptostroboides) 同屬新生代第三紀孑遺植物，為世界級之珍貴樹種。針對臺灣杉的抽出成分、生物活性、及其木材性質的研究，已累積相當多的成果；但就分子生物學的角度而言，對於參與臺灣杉生長、發育及二次代謝物的生合成相關酵素之研究，則是較少被論及。因此，本計畫即欲延續我們臺灣杉表現序列標籤基因庫所累積之研究成果，藉由所製備之 DNA 生物晶片進行基因表現差異分析，並配合蛋白質體學、基因轉殖及生物資訊學等系統生物學之研究策略，深入探討調控林木生長發育之機制，期能藉此縮短開花期、增加林木種子生成、加速林木之生長以及提高木材品質及產量等。此外，此研究成果亦可提供演化及保育之重要參考資料。<br> Abstract: Taiwania (Taiwania cryptomerioides Hayata) is one of the most economically important endemic tree species grown in Taiwan. Taiwania as well as Ginkgo biloba, Sequoiadendron giganteum and Metasequoia glyptostroboides are the “living fossil” from the Tertiary period of the Cenozoic era. To consider in the wood utilization and bioactivity points of view, there are several research results of Taiwania published in international scientific journals. However, less investigation of the importance of enzymes in the tree growth and development at a molecular level in Taiwania up to date. Therefore, in this proposed project, we will study on the functional genomics of Taiwania growth and development based on our established expression sequence tags (ESTs) library and the results of microarray analysis by functional genomic approaches. Coupling with proteomics, microarray analysis, transformation system and bioinformatics, we hope in the future to explore the genes and/or enzymes, which involved in the growth and development in Taiwania. Based on these efforts, it will obtain the genes associated with differential and metabolism to provide the useful information for the researches on tree physiology, tree improvement, evolution, and conservation in the future.臺灣杉DNA 生物晶片生長發育功能性基因體學Taiwania cryptomerioidesDNA microarraygrowthdevelopmentfunctional genomics