Wang C.-C.Fu C.-L.YAO-HSU YANGLo Y.-C.LI-CHIEH WANGYA-HUI CHUANGChang D.-M.BOR-LUEN CHIANG2020-07-012020-07-0120060969-7128https://scholars.lib.ntu.edu.tw/handle/123456789/507646Interleukin-1 (IL-1) is a proinflammatory cytokine and IL-1 receptor antagonist (IL-1ra) is a natural inhibitor that binds to IL-1 receptor type I without inducing signal transduction. It is suggested that IL-1 is required for allergen-specific T helper type 2 cell activation and the development of airway hyper-responsiveness (AHR), but the immunologic effect of exogenous IL-1ra in allergic asthma remains unclear. To examine the effect of IL-1ra on airway inflammation and immunoeffector cells in allergic asthma, recombinant adenovirus expressing human IL-1ra (Ad-hIL-1ra) was delivered intranasally into ovalbumin (OVA)-immunized mice. Single intranasal administration of Ad-hIL-1ra before airway antigen challenge in OVA-immunized mice significantly decreased the severity of AHR and reduced pulmonary infiltration of eosinophils and neutrophils. Suppression of IL-5 and eotaxin with concomitant enhancement of interferon gamma in bronchoalveolar lavage fluid was also noted in OVA-immunized mice by administration of Ad-hIL-1ra. In addition, histological studies showed that Ad-hIL-1ra was able to decrease OVA-induced peribronchial inflammation. Taken together, our results indicated that administration of Ad-hIL-1ra may have therapeutic potential for the immunomodulatory treatment of allergic asthma.[SDGs]SDG3adenovirus vector; eotaxin; gamma interferon; interleukin 1 receptor blocking agent; interleukin 5; ovalbumin; allergic asthma; animal experiment; animal model; animal tissue; antiinflammatory activity; article; controlled study; disease severity; drug megadose; eosinophil; female; gene expression; histopathology; immunomodulation; low drug dose; lung infiltrate; lung lavage; mouse; neutrophil chemotaxis; nonhuman; priority journal; viral gene delivery system; viral gene therapy; virus recombinant; Adenoviridae; Administration, Inhalation; Animals; Asthma; Bronchi; Bronchoalveolar Lavage Fluid; Chemokine CCL11; Chemokines, CC; Female; Gene Therapy; Genetic Engineering; Genetic Vectors; Hypersensitivity; Interferon Type II; Interferon-gamma; Interleukin 1 Receptor Antagonist Protein; Interleukin-5; Lung; Mice; Mice, Inbred BALB C; Models, Animal; Ovalbumin; Th2 Cells; Transduction, Genetic; Adenoviridae; Murinaejournal article10.1038/sj.gt.3302798167240922-s2.0-33748879109