2007-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660800摘要：近年來，利用轉殖防禦相關基因，以誘導作物產生抗病性之遺傳工程策略，已常被應用於病害管理。然而，為誘導出更持久且廣效之抗病性，且避免基因轉殖影響作物之正常生長發育，最關鍵的工作是應用具潛力之植物病害防禦基因與病菌誘導基因與啟動子。茄科青枯病為一複雜之重要土壤傳播性病害，基於抗病育種之困難性、抗病策略需求之迫切性及植物土壤傳播系統性病害抗病機制重要訊息嚴重缺乏等考量，本計畫選擇此病害為本計畫之研究對象，利用兩種研究策略研發植物防禦/病原菌誘導基因與啟動子。目前，利用subtractive cDNA microarray配合differential screening策略，我們已經找到一群在抗病品系與感病品系具差異性表現的基因及病原菌誘導基因。另外，我們已改良病毒誘發基因靜默載體，並在茄科作物建立此技術平台於根部系統性病害之系統性研究，現正用此系統分析選自array之基因及特定植物防禦基因在抗青枯病機制中扮演的角色，並將用此系統直接大量篩選番茄青枯病抗病的關鍵防禦基因。同時，我們正在分析可被青枯病菌誘導表現的基因啟動子及可能的cis-element。針對特定防禦/病菌誘導基因與啟動子，將進一步應用各種研究策略，探討它們在抗/感病反應中可能扮演的角色，並研發它們在作物保護及其他農業生技上之應用性。<br> Abstract: Transforming defense-related genes to induce plant resistance has become a common approach for disease management considering efficacy, efficiency, and reduced pesticide use. It is desired that the induced resistance should not only be durable and with broad spectrum, but also without deteriorating effect on plants. Thus, comprehensive information on genes involved in plant defense and resistance response would be highly desirable and necessary. We choose bacterial wilt of Solanaceaous crops as the case disease in this proposal as it is one of the most complex and serious crop diseases worldwide, and breeding for durable resistance for this disease is important and urgent. In addition, information on plant defense response to systemic infection of soilborne pathogens is very limited. We therefore intend to identify plant defense/inducible genes involved in tomato response to the infection of Ralstonia solanacerum, the causing agent of bacterial wilt. The approaches to be employed for the studies proposed herein include differential cDNA microarray hybridization and virus-induced gene silencing (VIGS). Furthermore, by combining various molecular and transgenic technologies, we are studying role(s) of selected defense/inducible genes in tomato disease-defense, as well as seek for potential applications of these genes and their promoters for crop protection/improvement. Through these studies, we hope to identify a group of agronomically important and useful genes and promoters that may benefit our future work on tomato or relevant crop improvement. The projected outputs of these studies include platform technology development, patents and intellectual properties, publications and personnel training. Therefore, we expect this could benefit both fundamental and applied agricultural research.茄科作物青枯病防禦基因病原菌誘導基因與啟動子基因靜默DNA晶片Solanaceousbacterial wiltdisease defensepathogen-induciblepromotervirus-induced gene silencingcDNA microarray