Huang, C.-Y.C.-Y.HuangChang, C.-W.C.-W.ChangTseng, Y.-J.Y.-J.TsengLee, J.J.LeeSung, P.-J.P.-J.SungSu, J.-H.J.-H.SuHwang, T.-L.T.-L.HwangCHANG-FENG DAIWang, H.-C.H.-C.WangSheu, J.-H.J.-H.Sheu2018-09-102018-09-102016http://www.scopus.com/inward/record.url?eid=2-s2.0-84992694446&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/397091Three new steroids, petasitosterones A and B (1 and 2) and a spirosteroid petasitosterone C (3), along with eight known steroids (4-11), were isolated from a Formosan marine soft coral Umbellulifera petasites. The structures of these compounds were elucidated by extensive spectroscopic analysis and comparison of spectroscopic data with those reported. Compound 3 is a marine steroid with a rarely found A/B spiro[4,5]decane ring system. Compounds 1-3 and 5 displayed inhibitory activity against the proliferation of a limited panel of cancer cell lines, whereas 2 and 5 exhibited significant anti-inflammatory activity to inhibit nitric oxide (NO) production. The inhibitory activities for superoxide anion generation and elastase release of compounds 1-11 were also examined to evaluate the anti-inflammatory potential, and 2-4 were shown to exhibit significant activities. ? 2016 by the authors.Anti-inflammatory activity; Cytotoxic activity; Soft coral; Steroid; Umbellulifera petasites[SDGs]SDG325alpha,8alpha epidioxy 24 methylcholesta 6,22 dien 3beta ol; 5alpha pregna 1,20 dien 3 one; 5alpha pregna 20 en 3 one; 5alpha,8alpha epidioxy 24 methylcholesta 6,22 dien 3beta ol; 5alpha,8alpha epidioxy 24alpha ethylcholesta 6 en 3beta ol; 5alpha,8alpha epidioxy 24alpha ethylcholesta 6,22 dien 3beta ol; 5alpha,8alpha epidioxycholesta 6 en 3beta ol; 5alpha,8alpha epidioxycholesta 6,22 dien 3beta ol; antiinflammatory agent; cytotoxic agent; doxorubicin; elastase; nitric oxide; petasitosterone A; petasitosterone B; petasitosterone C; steroid; superoxide; unclassified drug; antiinflammatory agent; antineoplastic agent; steroid; animal tissue; antiinflammatory activity; Article; biological activity; cancer cell destruction; cancer cell line; carbon nuclear magnetic resonance; cell proliferation; controlled study; coral; cytotoxicity; drug structure; enzyme release; human; human cell; IC50; nonhuman; proton nuclear magnetic resonance; structure analysis; Umbellulifera petasites; animal; Anthozoa; chemistry; drug effects; sea; structure activity relation; Taiwan; tumor cell line; Animals; Anthozoa; Anti-Inflammatory Agents; Antineoplastic Agents; Cell Line, Tumor; Oceans and Seas; Steroids; Structure-Activity Relationship; Taiwanjournal article10.3390/md141001802-s2.0-84992694446WOS:000387546400010