2021-02-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/665409"腸道黏膜是由上皮組織、菌相和免疫細胞所共同組成的複雜生態系統。腸道上皮細胞會製造黏液、產生抗微生物胜肽以 保護宿主。這些細胞藉由模式辨識受體直接感測微生物及其產物，進而整合這些訊號以調控腸道平衡。模式辨識受體的活化一般會促進發炎免疫反應，腸道上皮組織亦會產生抗發炎細胞激素來限制發炎反應。我們的初步實驗結果證明在AA小鼠的腸道淋巴結中，CD4+T細胞和CD8+T細胞會增加。此外，AA小鼠的腸道黏膜層產生較多的IL-17、IL-22及抗微生物胜肽，並且在腸道中有微生物菌相的不平衡。在本研究計畫，我們將會研究CBP磷酸化與腸道菌相對於黏膜免疫調節作用的相互影響。" "The mucosal surface of the gut is a complex ecosystem consisting of the epithelium, microbiota and immune cells. Intestinal mucous layer provides a primary physical and chemical barrier to protect the host against the invasion of harmful microorganisms and toxins. The mucus layers of gut are lined by epithelial cells which produced mucus and antimicrobial peptides (AMP) to protect the violation of microbiota to host. The integrity of intestinal barrier and gut homeostasis is controlled by epithelial cells, integrating multiple signals from direct sensing of microbes or microbe-derived products via Toll-like receptors (TLR), Nod-like receptors (NLR) and short-chain fatty-acid receptors.Since activation of pattern recognition receptors such as TLR and NLR, typically promotes pro-inflammatory innate responses, the intestinal epithelium produced anti-inflammatory cytokines and chemokines to limit inflammation through transmitting the signals to mucosal immune cells. Our preliminary data demonstrated that CD4+ and CD8 T+ cells were increased in mesenteric lymph nodes of AA mice. Moreover, AA mice produced more IL-17, IL-22 and AMP in the mucus layer and possessed a microbial imbalance in gut. In this proposal, the interplay of CBP phosphorylation and microbiome in mucosal immuno-modulation will be investigated."CBPCBP磷酸化菌相黏膜性免疫CBP phosphorylationmicrobiotamucosal immunity