江伯倫臺灣大學:免疫學研究所李坤珀Li, Kun-PoKun-PoLi2010-05-102018-07-092010-05-102018-07-092009U0001-2807200917593300http://ntur.lib.ntu.edu.tw//handle/246246/181822B-1 cells are a class of atypical B lymphocytes distinguished from conventional B-2 cells. It has been reported that the B-1 cells may play some roles in innate immunity, autoimmune diseases, and leukemia. Since B-1 cells are one of the most important sources of IL-10, we hypothesize that the B-1 cells may suppress the T cells activity and induce the generation of Tr1 cells. The mouse peritoneal cavity cells were analyzed and the B-1 cells were purified for following experiments. These B-1 cells could spontaneously secreted IL-10 and up-regulated the IL-10 production after LPS stimulation. The B-1 cells would inhibit proliferation of T cells through soluble factors. However, the B-1 cells could not efficiently induce the generation of IL-10-producing Treg cells in our system. In the contrast, the B-2 cell-induced Treg cells, “TofB2” cells, showed suppressive ability, and they presented Foxp3-CD25+CD62L+ phenotype and expressed high level of IL-10 and IL-4. In conclusion, the B-1 cells inhibited proliferation of T cells through soluble factors, and the B-2 cells join the immune modulation through inducing Treg cells generation. In different mechanisms, both B cell populations play regulatory roles in immune response. The studies may provide potent clinical application in treatments for autoimmune diseases.1. 摘要…………………………………………………………………………………i. Abstract……………………………………………………………………………ii. Contents…………………………………………………………………………iii. Contents of Figures………………………………………………………...…….iv. Introduction…………………………………..……………………………………1. Experimental Aims………………………………..………………………………9. Materials………………………………………………………………………….10. Methods…………………………………………………………………………..15. Results……………………………………………………………………………220. Discussion………………………………………………………………………...301. Figures……………………………………………………………………………392. References………………………………………………………………………63application/pdf5224644 bytesapplication/pdfen-USＢ-１淋巴球Ｂ-２淋巴球調節性Ｔ細胞介白素-１０B-1 LymphocytesB-2 LymphocytesRegulatory T cells (Treg)IL-10http://ntur.lib.ntu.edu.tw/bitstream/246246/181822/1/ntu-98-R96449005-1.pdf