Lai C.-S.Yang G.Li S.Lee P.-S.Wang B.N.Chung M.-C.Nagabhushanam K.Ho C.-T.MIN-HSIUNG PAN2019-07-122019-07-12201700218561https://scholars.lib.ntu.edu.tw/handle/123456789/4136233¡¬-Hydroxypterostilbene (trans-3,5-dimethoxy-3¡¬,4¡¬-hydroxystilbene) presents in Sphaerophysa salsula, Pterocarpus marsupium, and honey bee propolis and has been reported to exhibit several biological activities. Herein, we aimed to explore the chemopreventive effects of dietary 3¡¬-hydroxypterostilbene and underlying molecular mechanisms on colitis-associated cancer using the azoxymethane (AOM)/dextran sodium sulfate (DSS) model. 3¡¬-Hydroxypterostilbene administration effectively ameliorated the colon shortening and number of tumors in AOM/DSS-treated mice (3.2 ¡Ó 1.2 of the high-dose treatment versus 13.8 ¡Ó 5.3 of the AOM/DSS group, p < 0.05). Molecular analysis exhibited the anti-inflammatory activity of 3¡¬-hydroxypterostilbene by a significant decrease in the levels of inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-6 (IL-6) (p < 0.05). Moreover, dietary 3¡¬-hydroxypterostilbene also significantly diminished IL-6/signal transducer and activator of transcription signaling and restored colonic suppressor of cytokine signaling 3 levels in the colonic tissue of mice (p < 0.05). Collectively, these results demonstrated for the first time the in vivo chemopreventive efficacy and molecular mechanisms of dietary 3¡¬-hydroxypterostilbene against colitis-associated colonic tumorigenesis. ? 2017 American Chemical Society.3¡¬-hydroxypterostilbeneazoxymethanecolitisdextran sodium sulfateinflammation[SDGs]SDG3Immunology; Mammals; Nitric oxide; Sodium; Sodium sulfate; Sulfur compounds; Anti-inflammatory activity; azoxymethane; Chemopreventive effects; colitis; High-dose treatment; Inducible nitric oxide synthase; inflammation; Suppressor of cytokine signaling 3; Diseases; 3'-hydroxypterostilbene; antineoplastic agent; cyclooxygenase 2; interleukin 6; STAT3 protein; stilbene derivative; animal; carcinogenesis; colitis; colon tumor; complication; disease model; drug effects; genetics; human; Institute for Cancer Research mouse; male; metabolism; mouse; signal transduction; Animals; Anticarcinogenic Agents; Carcinogenesis; Colitis; Colonic Neoplasms; Cyclooxygenase 2; Disease Models, Animal; Humans; Interleukin-6; Male; Mice; Mice, Inbred ICR; Signal Transduction; STAT3 Transcription Factor; Stilbenesjournal article10.1021/acs.jafc.7b037122-s2.0-85033388512https://www.scopus.com/inward/record.uri?eid=2-s2.0-85033388512&doi=10.1021%2facs.jafc.7b03712&partnerID=40&md5=bb414fe08008d1b7036284e4cc2be528