楊台鴻臺灣大學：高分子科學與工程學研究所張家豪Chang, Chia-HaoChia-HaoChang2007-11-292018-06-292007-11-292018-06-292005http://ntur.lib.ntu.edu.tw//handle/246246/62878本實驗利用丁基鋰化合物在不同推電子溶劑(乙二胺、丁二胺及乙胺)催化下，改質高分子聚四氟乙烯(PTFE)，使其產生去氟烷化反應；利用FTIR-ATR、ESCA分析改質前後表面化學組成差異並測量接觸角以判斷親疏水性變化。 同時利用體外細胞培養的方法，來探討顆粒型神經細胞在改質前後PTFE的適合性，並且利用微流道技術設計細胞圖案，使PTFE產生親疏水性差異的表面。結果使用金相顯微鏡及掃描式電子顯微鏡觀察細胞生長。由結果得知，未改質前細胞無法貼附生長，在經由改質後之PTFE表面可以發現細胞群聚成球且生長突觸的現象。In this study, butyllithium was catalyzed by different electron-donating solvents (ethyldiamine, butyldiamine and ethylamine) for defluorination and alkylation to modify PTFE. FTIR-ATR and a ESCA results were used to analyze the composition of unmodified / modified surfaces and contact angles were measured to distinguish the variation of hydrophobicity. In vitro cerebellum cortical granule cells culturing was then used to test the biocompatibility of PTFE before and after modification, Microfluidic channels were also used to produce differential hydrophilic surfaces for cell patterning. Finally, a Metallurgical Microscope and a SEM were used to observe cell growing. According to the experimental results, cells could not adhere to unmodified PTFE surfaces. On the other hand, small populations of cells and synapse extension were observed on the modified PTFE surface.第一章 緒論．．．．．．．．．．．．．．．．．．．． 1 1-1 前言．．．．．．．．．．．．．．．．．．．．．． 1 1-2 神經細胞簡介．．．．．．．．．．．．．．．．．． 3 1-2-1 影響神經細胞生長的因素．．．．．．．．．．． 4 1-3 細胞圖案簡介．．．．．．．．．．．．．．．．．． 6 1-4 PTFE簡介．．．．．．．．．．．．．．．．．．． 8 1-4-1 PTFE化學改質簡介．．．．．．．．．．．．．．． 9 第二章 實驗流程與步驟．．．．．．．．．．．．．．． 11 2-1 實驗架構．．．．．．．．．．．．．．．．．．．． 11 2-2 實驗藥品．．．．．．．．．．．．．．．．．．．．． 12 2-3 實驗儀器．．．．．．．．．．．．．．．．．．．．． 15 2-4 PTFE表面改質．．．．．．．．．．．．．．．．．．． 19 2-4-1實驗設備．．．．．．．．．．．．．．．．．．．． 21 2-4-2實驗步驟．．．．．．．．．．．．．．．．．．． 22 2-5 細胞圖案．．．．．．．．．．．．．．．．．．．．． 23 2-5-1 實驗裝置．．．．．．．．．．．．．．．．．．．． 23 2-6 材料表面鑑定．．．．．．．．．．．．．．．．．．． 24 2-6-1 接觸角實驗(Contact Angle)．．．．．．．．．．． 24 2-6-2衰減全反射傅立葉紅外光譜儀(ATR-FTIR)．．．．．． 25 2-6-3化學分析電子光譜(ESCA)．．．．．．．．．．．．． 26 2-7 神經細胞培養．．．．．．．．．．．．．．．．．． 27 2-7-1 試藥配製．．．．．．．．．．．．．．．．．．．． 28 2-7-2 材料前處理．．．．．．．．．．．．．．．．．．． 31 2-7-3 塗佈聚離胺酸．．．．．．．．．．．．．．．．．． 31 2-7-4顆粒型神經細胞初代培養．．．．．．．．．．．．． 32 2-8 掃描式電子顯微鏡觀察(SEM)．．．．．．．．．．．． 33 2-8-1 細胞固定(Fixation)．．．．．．．．．．．．．．． 34 2-8-2 臨界點乾燥(Critical Point Dryer)．．．．．．．． 35 2-8-3 樣品鍍金．．．．．．．．．．．．．．．．．．．． 36 第三章 結果與討論．．．．．．．．．．．．．．．．． 37 3-1 PTFE表面改質分析．．．．．．．．．．．．．．．．． 37 3-1-1 接觸角實驗(Contact Angle)．．．．．．．．．．．．37 3-1-2衰減全反射傅立葉紅外光譜儀(ATR-FTIR)．．．．．． 38 3-1-3化學分析電子光譜(ESCA)．．．．．．．．．．．．． 40 3-2 神經細胞培養．．．．．．．．．．．．．．．．．．． 42 3-2-1顆粒型神經細胞初代培養．．．．．．．．．．．．． 42 3-3 細胞圖案．．．．．．．．．．．．．．．．．．．．． 43 第四章 結論．．．．．．．．．．．．．．．．．．．． 44 第五章 未來展望．．．．．．．．．．．．．．．．．． 45 第六章 參考文獻．．．．．．．．．．．．．．．．．． 47 附錄．．．．．．．．．．．．．．．．．．．．．．．． 665671192 bytesapplication/pdfen-US小腦顆粒型神經細胞聚四氟乙烯丁基鋰推電子溶劑親疏水性細胞圖案微流道去氟反應烷化反應cerebellum cortical granule cellspolytetrafluoroethenebutyllithiumelectron-donating solventhydrophobicitycellular patternmicrofluidic channeldefluorinationalkylationthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/62878/1/ntu-94-R92549023-1.pdf