王尚禮Chia-Hsuan Chang張嘉軒2025-11-142025-11-142025https://scholars.lib.ntu.edu.tw/handle/123456789/733692https://ntu.primo.exlibrisgroup.com/permalink/886NTU_INST/14poklj/alma991039401169404786獎項：校長獎；指導教授：王尚禮Coffee grounds, a common organic waste, have potential for soil amendment but may increase soil CO₂ and N₂O emissions. However, the direct addition of coffee grounds may enhance soil emissions of carbon dioxide (CO₂) and nitrous oxide (N₂O). Earthworms hav e been reported to reduce N₂O emissions and enhance organic matter stability. This study investigates the effects of earthworms on greenhouse gas emissions and carbon nitrogen transformations during coffee ground decomposition. Based on previous studies, b oth earthworms and coffee grounds individually increase CO₂ and N₂O emissions. This study hypothesized that the interaction between earthworms and coffee grounds would mitigate N₂O emissions by enhancing organic matter stability and improving soil aeration . To test this hypothesis, an incubation experiment was conducted to measure greenhouse gas emissions, nitrogen transformations, and organic matter changes. The results showed that coffee ground addition increased N₂O and CO₂ emissions, likely due to a priming effect caused by its high organic content. However, contrary to our hypothesis, earthworms further elevated emissions. This may be due to their enhancement of nitrogen mineralization and stimulation of denitrification. Additionally, earthworms may hav e consumed labile organic carbon in the early stage of the experiment, further promoting nitrogen loss. Despite increasing greenhouse gas emissions, earthworms contributed to soil carbon stabilization, which may support long term carbon sequestration. These findings highlight the complex role of earthworms in organic matter decomposition and their potential implications for soil carbon and nitrogen dynamics.咖啡渣是現代社會常見的有機廢棄物，具有很高的再利用價值，在土壤改良與有機肥應用方面表現良好。然而，直接添加咖啡渣可能因為礦化作用而促進土壤二氧化碳 (CO 2 排放，且有機質進入土壤中的促發效應也會造成氧化亞氮 (N 2 O)排放，對環境產生負面影響。有研究指出，蚯蚓能減少 有機質分解時的氧化亞氮排放並提高有機質的穩定性，因此，本研究以蚯蚓與咖啡渣的添加作為變因，探討蚯蚓對咖啡渣分解過程中溫室氣體排放及碳氮轉化的影響。根據前人文獻與預試驗結果，已知單獨加蚯蚓與單獨加咖啡渣皆會增加氧化亞氮與二氧化碳的排放，本研究假設以蚯蚓處理咖啡渣 相對於單獨加咖啡渣或蚯蚓，能減少氧化亞氮的排放，其可能是因為蚯蚓 透過改變土壤有機質穩定程度，間接影響氮循環，且蚯蚓增進土壤通氣性，進而減少氧化亞氮的排放。 為驗證此假設，本研究透過孵育試驗測量土壤溫室氣體排放、氮物種變化與有機質形態。 結果顯示咖啡渣的添加確實促進氧化亞氮和二氧 化碳的排放，這可能是由於咖啡渣的高有機質含量造成促發效應。然而，與假設相反的是，蚯蚓處理咖啡渣反而進一步提升了 氧化亞氮與二氧化碳在 咖啡渣分解過程中的排放。 可能的機制包括：蚯蚓促進了咖啡渣中氮的礦化，加速氮循環向脫氮的方向進行；此外，蚯蚓可能在試驗初期第一週消耗土壤中易分解的有機碳，進一步增強脫氮作用。然而，儘管蚯蚓的添加增加了溫室氣體排放，但同時也提升了土壤碳的穩定性，可能有助於長期碳封存，對土壤碳匯產生潛在的正向影響。coffee groundsearthwormsgreenhouse gasessoil organic carbonnitrogen cycle咖啡渣蚯蚓溫室氣體土壤有機質氮循環thesis