Wu, Cyun-MingCyun-MingWuLi, Hao-YunHao-YunLiWEN-YI LIHsu, Chih-NengChih-NengHsuWEI-SHUN YANGGWO-TSANN CHUANGChe, Tony Pan-HouTony Pan-HouCheLee, Tung-YuanTung-YuanLeeLee, Hsiao-LinHsiao-LinLeeHee, Siow-WeySiow-WeyHeeLee, Jiin-HorngJiin-HorngLeeLiao, DanielDanielLiaoLiao, Karen Chia-WenKaren Chia-WenLiaoHsuan, Chin-FengChin-FengHsuanLiu, Ya-WenYa-WenLiuChu, Shao-LunShao-LunChuYI-CHENG CHANG2024-12-232024-12-232024-12-06https://scholars.lib.ntu.edu.tw/handle/123456789/7242332-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.enHealth SciencesMetabolismMetabolomicsjournal article10.1016/j.xpro.2024.10347839644497