2013-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/645597摘要：齲齒仍居口腔疾病高發生率。目前填補材料仍難免齲齒再侵襲。齲齒是經由反覆酸化、再礦化、脫礦化最後形成窩洞，其中細菌的副產物及膠原蛋白酶佔有重要腳色以溶解牙齒無機質及有機質。兒茶素(表沒食子兒茶酚沒食子酸)為茶葉茶多酚裡面的主成分，具有多樣重要的生理作用如抗氧化、抗發炎、抗癌、抗菌、抗酸蝕等。本研究團隊過去一年與茶改場場長陳右人教授進行台灣茶成分及牙齒抗酸蝕研究，前導實驗確有顯著抗酸蝕及促進再礦化潛力。因此將結合兒茶素抗菌能力，研發含兒茶素複合材料來達到抗齲齒。本計畫擬分三年進行，第一年開發抗齲齒兒茶素複合樹脂，方向以聚胺酯丙烯酸樹脂作為基質，以二異氰酸鹽等加強其交聯網狀結構，利用兒茶素氫氧基，接枝到寡聚物上。評估其組成與機械性質與初步抗菌效果後，進入第二年，測試各種材料之細胞相容性、抵抗脫礦化、再礦化及生物膜抗菌/抑菌等效果，第三年動物實驗結合微電腦斷層掃描，將探討與驗證本材料於修復性牙本質生成能力。本計畫結合材料研發、生物膜反應試驗、細胞組織修復及動物實驗，將材料組成、生物相容性及抗菌性最佳化，預期可預防齲齒及應用於深度窩洞覆髓材，未來更可將所研發的材料系統應用於其他生醫材料的改良。<br> Abstract: Caries remains high incidence of oral disease. Present filling materials are hard to avoidthe re-invasion of caries. Dental caries is caused by acidification, remineralization,demineralization and form the cavity finally. By-products of bacterials combined withcollagenase play an important role to dissolve inorganic and organic matrix of teeth.EGCG (epigallocatechin gallate), the main component of tea polyphenols, exhibits variousimportant physiological functions such as antioxidant, anti-inflammatory, anti-cancer,anti-bacterial and anti-erosion. Over the past year, our research team have been cooperatingwith the head of “Tea Research and Extension Station”, Prof. 陳右人 for Taiwan teacomposition and teeth anti-erosion research. Pilot studies indicated that EGCC showssignificant resistance to erosion and promote remineralization of teeth. Thus, we plan todevelop EGCG-containing composite materials by the combination of EGCG antibacterialcapabilities to achieve caries prevention.We plan to conduct a three-year research project. The first year is aim to developanti-caries EGCG-containing composite resin. The urethane-based acrylic resin will act asthe matrix. We will enhance the cross-linking structure of the resin matrix by addingdiisocyanate derivatives. The use of EGCG phenolic hydroxyl group will be branched intothe oligomer performing the anti-bacterial function. And we will optimize the composition,mechanical properties and the initial antibacterial effect. The second year, we will rule outthe high-cytotoxicity materials by MTT assay and agar diffusion test. De-mineralization/remineralization and biofilm/antibacterial effects will be tested for the evaluation of cariesprevention in vitro. The third year, we will incorporate micro-CT evaluation and histologicalexamination of animal study to gain more insight into the cell-biomaterials interaction inrelation to the reparative dentin formation or pulp-dentin regeneration.This project is designed to carry us through a complete process (development ofmaterials, biofilm reactor experiments, cell-tissue interaction and animal study) fromunderstanding the mechanisms underlying cariosatic effects to the developed materials andto in vivo animal study to test its efficacy. We expect the novel EGCG-containing compositematerials can prevent caries and even achieve pulp-dentin regeneration. We also hope thenew material properties and its biocompatibility will be further applied to improvementsusages biomedical materials.抗齲齒兒茶素聚胺酯丙烯酸複合樹脂生物膜反應器微電腦斷層掃描anti-cariesEGCG (epigallocatechin gallate)urethane-based acrylic compositebiofilm reactormicro-CT (micro-computed tomography)