Hui, Y. Y.Y. Y.HuiSu, L. J.L. J.SuChen, O. Y.O. Y.ChenYIT-TSONG CHENLiu, T. M.T. M.LiuChang, H. C.H. C.Chang2018-09-102018-09-102014http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000338506300002&KeyUID=WOS:000338506300002http://scholars.lib.ntu.edu.tw/handle/123456789/388484Nanodiamonds containing high density ensembles of negatively charged nitrogen-vacancy (NV-) centers are promising fluorescent biomarkers due to their excellent photostability and biocompatibility. The NV-?' centers in the particles have a fluorescence lifetime of up to 20ns ...ns, which distinctly differs from those (<10 ...ns) of cell and tissue autofluorescence, making it possible to achieve background-free detection in vivo by time gating. Here, we demonstrate the feasibility of using fluorescent nanodiamonds (FNDs) as optical labels for wide-field time-gated fluorescence imaging and flow cytometric analysis of cancer cells with a nanosecond intensified charge-coupled device (ICCD) as the detector. The combined technique has allowed us to acquire fluorescence images of FND-labeled HeLa cells in whole blood covered with a chicken breast of ?0.1-mm thickness at the single cell level, and to detect individual FND-labeled HeLa cells in blood flowing through a microfluidic device at a frame rate of 23 ...Hz, as well as to locate and trace FND-labeled lung cancer cells in the blood vessels of a mouse ear. It opens a new window for real-time imaging and tracking of transplanted cells (such as stem cells) in vivo.[SDGs]SDG3fluorescent dye; nanodiamond; animal; Bagg albino mouse; cancer transplantation; cell tracking; chemistry; chicken; flow cytometry; fluorescence microscopy; HeLa cell line; human; metabolism; microfluidic analysis; tumor embolism; Animals; Cell Tracking; Chickens; Flow Cytometry; Fluorescent Dyes; HeLa Cells; Humans; Mice, Inbred BALB C; Microfluidic Analytical Techniques; Microscopy, Fluorescence; Nanodiamonds; Neoplasm Transplantation; Neoplastic Cells, Circulatingjournal article10.1038/srep055742-s2.0-84903974191WOS:000338506300002