謝文陽2006-07-252018-06-282006-07-252018-06-282003http://ntur.lib.ntu.edu.tw//handle/246246/14821本研究自二仁溪、後勁溪、愛河和高屏溪採集河口水樣,進行其汞耐受性細 菌的計數、分離與鑑定之探討。採得之河口水,利用含不同濃度二價汞離子的 PY 液體培養基培養,配合最可能計數(most-probable-number counts,略稱MPN counts)法,所估得菌數多介於104-107 cells/L 間。從各MPN 培養系列中共純化 出34 株具汞耐受能力的菌株。根據革蘭氏反應、細胞外型、對氯化鈉的需求等 表型特性之差異, 可將這34 株分離株分為九群, 其中七群分別屬於 Acinetobacter 、Alteromonas 、Bacillus 、Hyphomonas 、Lactobacillus 、 Pseudoalteromonas 和Pseudomonas;另外兩個尚未鑑定至屬的菌群,分別包含 產生橙或黃色菌落的革蘭氏陽性需鹽性細菌及產生白色半透明或不透明菌落的 革蘭氏陽性需鹽性細菌。Mercury-tolerant bacteria distributed in estuarine water samples collected from Er-ren River, Hou-Jien River, Love River and Kao-ping River were enumerated by most-probable-number (MPN) counts. The counting values generally ranged from 104 to 107 cells/L. Thirty-four strains of mercury- tolerant bacteria were isolated from the MPN tubes. They were classified into nine groups according to the characterization tests, including Gram reaction, glucose fermentation, motility and NaCl requirement. Seven groups included strains that were identified as members of Acinetobacter, Alteromonas, Bacillus, Hyphomonas, Lactobacillus, Pseudoalteromonas and Pseudomonas, respectively. The other two groups included halophilic, Gram-positive strains that were not identified to the generic level; one group produced orange or yellow colonies on agar plates while colonies of the other group were white (opaque or translucent).application/pdf122156 bytesapplication/pdfzh-TW國立臺灣大學海洋研究所汞耐受性細菌mercury-tolerant bacteria污染河口水中汞耐受性細菌之研究reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/14821/1/912313B002327.pdf