HSIU-HUI SHIHHu, C. W.C. W.HuWang, C. S.C. S.Wang2009-07-012018-07-062009-07-012018-07-06200301758659http://ntur.lib.ntu.edu.tw//handle/246246/161733https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038138010&doi=10.1046%2fj.1439-0426.2003.00353.x&partnerID=40&md5=aacdcdc57333f2dcd63d1db1ea8e5405A nucleic acid probe for the Herpesvirus anguillae (HVA) Taiwan isolate was constructed using recombinant DNA techniques. This probe consisted of a specific viral DNA fragment (1550 bp) generated by digestion of HVA DNA with the restriction enzyme HindIII, and labeled non-radioactively with digoxigenin (DIG). The probe was used to detect the HVA genome from HVA-infected cell cultures and tissue specimens prepared from infected cels, using either dot blot or in situ hybridizations.application/pdf182160 bytesapplication/pdfen-USdetection method; eel; genetic analysis; viral disease; Anguilla anguilla; Anguilliformes; Herpesviridaejournal article10.1046/j.1439-0426.2003.00353.x2-s2.0-0038138010http://ntur.lib.ntu.edu.tw/bitstream/246246/161733/1/10.pdf