Chiu K.C.LEE-MING CHUANGRyu J.M.Tsai G.P.Saad M.F.2020-06-012020-06-0120000021-972Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0034457994&doi=10.1210%2fjc.85.6.2178&partnerID=40&md5=fbcca64dfdbd192c00f2bf0326bc21a2https://scholars.lib.ntu.edu.tw/handle/123456789/495687Mutations of the hepatic nuclear factor-1α (HNF-1α) gene have been found in patients with maturity-onset diabetes of the young. We examined the relation between the I27L polymorphism of HNF-1α and insulin sensitivity and β-cell function assessed by a hyperglycemic clamp. This study included 52 healthy glucose-tolerant and normotensive subjects (age, 19-40 yr; body mass index, 17.58-35.61 kg/m2; waist/hip ratio, 0.65-1.03). We identified 19 LL subjects, 24 IL, and 9 II subjects. No difference was noted in the demographic features among the three genotypes. The LL group had the highest postchallenge insulin levels at 30 and 90 min (P = 0.038 and P = 0.015, respectively) and also the highest insulin area under curve (P = 0.009) among the three genotypes. The LL group was more insulin resistant than the IL and II groups (P = 0.042 for insulin sensitivity index). After adjusting for age, gender, obesity, and ethnicity, the I27L polymorphism was an independent determinant of the insulin sensitivity index (P = 0.001). However, it had no impact on either the first or second phase insulin response. Therefore, we conclude that the I27L polymorphism is associated with insulin resistance, but not β-cell function. The mechanism of this association is unclear, but HNF-1α may play a role in regulating hepatic glucose metabolism.[SDGs]SDG3nuclear factor I; adult; amino acid metabolism; article; body mass; female; gene expression regulation; genetic polymorphism; genetic regulation; glucose metabolism; human; hyperglycemia; insulin resistance; insulin sensitivity; major clinical study; male; non insulin dependent diabetes mellitus; pancreas islet beta cell; priority journal; promoter region; Adult; Amino Acid Substitution; Blood Glucose; Blood Pressure; Body Constitution; Body Mass Index; DNA-Binding Proteins; Ethnic Groups; Female; Glucose Clamp Technique; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Insulin; Insulin Resistance; Lipids; Male; Nuclear Proteins; Polymorphism, Genetic; Transcription Factorsjournal article10.1210/jc.85.6.2178108524492-s2.0-0034457994