Twu, Yuh-ChingYuh-ChingTwuChou, Ming-LunMing-LunChouYu, Lung-ChihLung-ChihYu2009-08-052018-07-062009-08-052018-07-0620030006291Xhttp://ntur.lib.ntu.edu.tw//handle/246246/163216https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037432612&doi=10.1016%2fS0006-291X%2803%2900443-1&partnerID=40&md5=8bd395128f3e9f5c4c99a138b3ff4224The I antigen and its precursor, the i antigen, are carbohydrate structures and are found on the surface of most mammalian cells. Conversion of the i to the I structure requires I β-1,6-N-acetylglucosaminyltransferase activity. The present investigation demonstrates a novel transcript form expressed from the mouse I locus and elucidates the molecular genetics and the genomic organization of the mouse I locus. The mouse I locus was demonstrated to express three transcript forms, one newly identified and two previously reported, which have a different exon 1 but identical exons 2 and 3. The three transcripts were shown to express differentially in various mouse tissues, and all their protein products demonstrated GlcNAc-transferring activity in enzyme function assay. The molecular genetics proposed for the mouse I locus shows that it is homologous to the human I locus. It has been established recently that a defect in the human I locus may lead to the development of congenital cataracts. It was demonstrated that the mouse and the human I transcripts expressed in the epithelium cells of the mouse and human lens, respectively, are homologous forms. © 2003 Published by Elsevier Science (USA).application/pdf821730 bytesapplication/pdfen-USI locus; IGnT; Lens; β-1,6-N-acetylglucosaminyltransferasebeta 1,6 n acetylglucosaminyltransferase; blood group I antigen; n acetylgalactosamine; protein; transferase; unclassified drug; animal cell; article; congenital cataract; controlled study; enzyme activity; epithelium cell; gene expression; gene locus; genetic organization; genetic transcription; genome; human; male; molecular genetics; mouse; mouse strain; nonhuman; nucleotide sequence; priority journal; protein function; sequence homology; Mammaliajournal article10.1016/S0006-291X(03)00443-1126704912-s2.0-0037432612http://ntur.lib.ntu.edu.tw/bitstream/246246/163216/1/04.pdf