Yoshida, MitsunoriMitsunoriYoshidaSano, SotaroSotaroSanoJUNG-YIEN CHIENFukano, HanakoHanakoFukanoSuzuki, MasatoMasatoSuzukiAsakura, TakanoriTakanoriAsakuraMorimoto, KozoKozoMorimotoMurase, YoshiroYoshiroMuraseMiyamoto, ShigehikoShigehikoMiyamotoKurashima, AtsuyukiAtsuyukiKurashimaHasegawa, NaokiNaokiHasegawaPO-REN HSUEHMitarai, SatoshiSatoshiMitaraiAto, ManabuManabuAtoHoshino, YoshihikoYoshihikoHoshino2021-04-232021-04-232021-0223523964https://scholars.lib.ntu.edu.tw/handle/123456789/557665The clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group of emerging non-tuberculosis mycobacteria (NTM), is increasing. M. abscessus subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas M. abscessus subsp. massiliense is generally susceptible. Therefore, rapid and accurate discrimination of macrolide-susceptible MABC subgroups is required for effective clinical decisions about macrolide treatments for MABC infection. We aimed to develop a simple and rapid diagnostic that can identify MABC isolates showing macrolide susceptibility.enATS/ERS/ESCMID/IDSA guideline; DNA chromatography; Drug susceptibility; Non-tuberculosis mycobacteria; Subspecies discrimination; Whole genome sequencing[SDGs]SDG3[SDGs]SDG10[SDGs]SDG16azithromycin; clarithromycin; erythromycin; macrolide; RNA 16S; antiinfective agent; DNA; macrolide; antibiotic resistance; Article; bacterial strain; bacterium isolate; chromatography; DNA extraction; DNA sequence; drug sensitivity; genetic marker; genotype; limit of detection; multiplex polymerase chain reaction; Mycobacterium abscessus; nonhuman; phylogeny; polymerase chain reaction; sensitivity and specificity; sequence analysis; Taiwan; whole genome sequencing; bacterial gene; chromatography; classification; drug effect; genetics; human; microbial sensitivity test; Mycobacterium abscessus; procedures; Anti-Bacterial Agents; Chromatography; DNA; Drug Resistance, Bacterial; Genes, Bacterial; Humans; Macrolides; Microbial Sensitivity Tests; Mycobacterium abscessus; Phylogeny; Polymerase Chain Reactionjournal article10.1016/j.ebiom.2020.103187334464752-s2.0-85100106440https://scholars.lib.ntu.edu.tw/handle/123456789/550019