謝國煌臺灣大學:高分子科學與工程學研究所林育永Lin, Yu-YungYu-YungLin2010-05-122018-06-292010-05-122018-06-292009U0001-2007200920043300http://ntur.lib.ntu.edu.tw//handle/246246/183168幾丁聚醣(Chitosan)是近年來極具發展潛力的生醫材料，具有生物可分解性、生物相容性及抗菌性，且其在酸性環境下會形成帶正電的聚電解質，可以很容易的和其他帶負電的聚電解質或藥物(如生長因子等)形成錯合物，藉而增進材料的性質，因此應用極為廣泛，常被用在骨組織再造、藥物釋放、創傷敷料等應用。而聚麩胺酸(γ-PGA)則為近幾年開發成功，全天然生物可分解性高分子，具有高度之親水、保水能力，有生物凝膠及保健食品等應用。 本研究即利用不同比例的幾丁聚醣在酸性環境下，與聚麩胺酸形成聚錯合物(polyelectrolyte complex)，在冷凍乾燥後得到具有孔洞的支架材料，對此材料做機械性質、熱性質、生物性質等的測試，最後做動物實驗將此生醫材料應用在創傷敷料上。由傷口的巨觀上來看，跟對照組相比，此幾丁聚醣/聚麩胺酸複合支架對於傷口的癒合有顯著的幫助。由傷口的組織切片來看，幾丁聚醣對發炎細胞有抑制的作用，且對於纖維母細胞的增生有刺激的效果，而聚麩胺酸則是能增進上皮細胞的生長。綜合以上，本幾丁聚醣/聚麩胺酸複合支架是極具潛力的創傷敷料。Chitosan is a biomaterial that has been studied for years. It is biodegradable, biocompatible and has great antibacterial ability. Chitosan can dissolve in acid environment to become a cationic polyelectrolyte though can easily form polyelectrolyte complexes (PECs) with other anionic polyelectrolytes. Through this manner, the properties of chitosan can be enhanced, so chitosan is widely applied in bone tissue regeneration, drug delivery systems and wound dressings. Poly(γ-glutamic acid) (γ-PGA) is also a natural biodegradable polymer. It is well-known for its highly hydrophilicity, and is usually applied as bioglue. In this research, various types of scaffolds were prepared at different repeating unit ratios of chitosan and γ-PGA. The mechanical property, thermal property, and some biological properties were examined for the chitosan/γ-PGA composite scaffolds. At last, animal test was conducted to evaluate the possibility that our scaffold could be a potential wound dressing material. Results of wound closure observation showed that chitosan/γ-PGA composite scaffolds could accelerate the process of wound healing. From histological results, chitosan could suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation while γ-PGA could enhance epithelial migration. Thus, chitosan/γ-PGA composite scaffolds could be potential wound dressing materials.誌謝 I文摘要 IIbstract IIIontents IVist of tables VIist of figures VIIhapter 1 Introduction 1hapter 2 Literature review 3.1 Introduction of chitosan 3.1.1 Applications of chitosan 5.1.2 Chitosan for bone tissue engineering 13.1.3 Chitosan for drug delivery systems 15.1.4 Chitosan for wound healing application 19.2 Introduction of γ-PGA 21.3 Process of wound healing 24.3.1 Inflammation 26.3.2 Fibroplasia 27.3.3 Remodeling 27hapter 3 Materials and Equipments 29.1 Materials 29.2 Equipments 30hapter 4 Experiments 31cheme of the research 31.1 Preparation of chitosan/γ-PGA scaffolds 32.2 Chracterization of the scaffolds 34.2.1 Fourier-Transformed Infrared spectra 34.2.2 Compressive modulus 34.2.3 Thermogravimetric Analysis (TGA) 34.2.4 Morphology of scaffolds by Scanning electron microscopy 34.2.5 Water absorption test 35.3 Biological properties 35.3.1 In vitro biodegradation study 35.3.2 Antibacterial assessment 36.3.3 Biocompatibility test 37.3.4 Cell activity analysis by MTT assay 37.3.5 Cell morphology on scaffolds by SEM 37.4 Assessment for wound dressing application 38.4.1 Implant sdudy 38.4.2 Histological examination 38.5 Statistical analysis 39hapter 5 Results and Discussions 40.1 Characterization of the scaffolds 40.1.1 FTIR analysis 40.1.2 Thermal analysis 41.1.3 Water absorption 43.1.4 Morphology of porous scaffolds 44.1.5 Compressive modulus 45.2 Biological properties 46.2.1 In vitro biodegradation study 46.2.2 Cell morphology and activity on the scaffolds. 47.2.3 Antibacterial assessment 49.3 Implant study of wound dressing application 51.3.1 Wound closure observation 51.3.2 Histological observation 53hapter 6 Conclusions 66eferences 67application/pdf3113915 bytesapplication/pdfen-US幾丁聚醣聚麩胺酸支架創傷敷料chitosanγ-PGAscaffoldwound dressingthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/183168/1/ntu-98-R96549009-1.pdf