醫工所Chang, Chia-ChenChia-ChenChangLin, ShenhsiungShenhsiungLinChu-Su, YuYuChu-SuLin, Chii-WannChii-WannLin2012-07-132018-06-292012-07-132018-06-292011http://ntur.lib.ntu.edu.tw//handle/246246/241977In this paper, an improvement method for detection of tumor marker with a label-free biosensor is reported. The polyethylene glycol PEG-modified chitosan hydrogel as a biosensing interfacial architecture was formed on the gold surface and its potential was evaluated with a quartz crystal microbalance QCM. This hydrogel was modified to increase its negative charges and then use electrostatic interactions with the positive charges of the amine surfaces . The biosensing application was investigated by use of the antibody antigen model system of carbohydrate antigen 15-3 CA15-3, an important indicator in the prognosis of breast cancer. Compared to the covalent immobilization method, the QCM biosensor with the proposed immobilization showed improved sensitivity. In addition, the regeneration of the developed immunosensor was obtained after the release by rinsing the sensor with glycine/HCl solution. Based on these results, it was believed that such a three-dimensional polyelectrolyte multilayer can be applied to the development of immunoassays.en-USQuartz Crystal MicrobalancePolyethylene GlycolChitosan[SDGs]SDG3Aliphatic compounds; Amino acids; Antigens; Biosensors; Carbohydrates; Chitosan; Crystals; Diagnosis; Hydrogels; Multilayers; Polyelectrolytes; Polyethylene glycols; Polyethylenes; Quartz; Tumors; Antibody-antigen; Biosensing applications; Covalent immobilization; Improvement methods; Interfacial architecture; Label-free biosensor; Modified chitosan; Polyelectrolyte multilayer; Quartz crystal microbalances10.1166/sl.2011.14882-s2.0-83255192552WOS:000288645200083