2016-01-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/705078摘要：本臺拉立三方合作計畫共同擬定的合作計畫內容，將探討幹細胞與癌症幹細 胞對於奈米粒的反應，期望能發展具有生物相容性的奈米粒作為細胞標定，並建 立一評估奈米粒對幹細胞影響的平台。基於合作三方的專長，本計畫擬探討的奈 米粒為新穎可分解高分子被覆之超順磁性氧化鐵奈米粒與氧化鋅量子點；而細胞 則以間葉幹細胞與乳癌幹細胞為主，並經由合作彼此提供幹細胞與奈米粒。臺灣 方面研究人員負責的部分為：新穎可分解高分子被覆之超順磁性氧化鐵奈米粒與 氧化鋅量子點的合成與提供，及大鼠脂肪間葉幹細胞對奈米粒的反應及分化的影 響。第一年我們擬製備新穎之氧化鐵與氧化鋅奈米粒，測量可分解高分子被覆後 超順磁性與螢光效率的變化與時間的效應，分析奈米粒對大鼠脂肪間葉幹細胞的 標定效率、毒性、細胞行為與分化特性的變化，並探討細胞培養基材對於奈米粒 -細胞交互作用的影響；第二年擬將上述可分解高分子進一步與疏水性藥物結 合，分析藥物釋放的情形，並探討對間葉幹細胞特性的影響，期間並提供新穎奈 米粒及結合藥物之新穎奈米粒予合作方，各進行奈米粒標定幹細胞之紅外光譜偵 測(拉脫維亞方)與乳癌幹細胞的標定與治療效果(立陶宛方)之相關研究；第三年 擬以動物模式評估新穎奈米粒的標定效率與治療潛力。<br> Abstract: This TAIWAN –LATVIA –LITHUANIA cooperation project proposal is based on the individual expertise, mutual interests and agreement of the research contents from each party. We aim to study the response of stem cells to nanoparticle (NP) treatment, to develop biocompatible NPs for stem cell labeling and treatment, and to establish a platform to evaluate the effects of NPs on stem cell behavior. The NPs are self-synthesized novel biodegradable polymer-stabilized superparamagnetic ion oxide (Fe3O4) NPs and zinc oxide (ZnO) QDs. The stem cells include mesenchymal stem cells (MSCs) and breast tumor cancer stem-like cells (CSCs). The research teams will exchange these NPs and stem cells for collaborative studies. In Taiwan, we are focusing on preparing and providing the novel biodegradable polymer-stabilized Fe3O4 NPs and ZnO QDs and the response of rat adipose-derived MSCs to these NPs. In the first year, we will synthesize novel biodegradable polymer-stabilized Fe3O4 NPs and ZnO QDs via chemical methods, analyze the time-dependent changes in superparamagnetic and fluorescent properties, study the uptake and influence of these NPs in MSCs, and examine the effect of substrates on NP-stem cell interaction. In the second year, we will further incorporate hydrophobic drugs to the NPs and study the drug release and effects on MSCs. We will offer our novel NPs to the Latvian team regarding the use of FT-IR to detect the change of MSCs upon NP exposure and to the Lithuanian team regarding the theranostic potential of NPs on CSCs and cancer treatment. In the third year, we will evaluate the tracking abilities and therapeutic functions of MSCs by in vivo studies.間葉幹細胞可分解高分子奈米粒紅外線光譜癌症幹細胞mesenchymal stem cells (MSCs)biodegradable polymersnanoparticles(NPs)Fourier transform infrared spectroscopy (FT-IR)cancer stem-like cells(CSCs).