Chen, Yun YuanYun YuanChenLu, Chi TeChi TeLuTsai, Mei HuaMei HuaTsaiYang, Cheng FenCheng FenYangShu, Pei YunPei YunShuKEVIN CHIA-WEN WUChen, Jen WeiJen WeiChenHung, Chi MingChi MingHungWei, Sheng TangSheng TangWeiHou, Sheng MouSheng MouHouPEI-JER CHEN2023-06-282023-06-282022-05-0100221899https://scholars.lib.ntu.edu.tw/handle/123456789/633229Background: Evidence for mitigation of transfusion-transmitted dengue informed by surveillance data is lacking. In this study, we evaluated the risk of positive dengue viral (DENV) ribonucleic acid (RNA) from blood transfusions during a large outbreak in Taiwan. Methods: Serum collected from blood donors living in districts experiencing the dengue epidemic were tested for DENV RNA using a qualitative transcription-mediated nucleic acid amplification assay (TMA). The TMA-reactive specimens were further tested for immunoglobulin (Ig)M and IgG antibodies, nonstructural protein 1 (NS1) antigen, and viral RNA by reverse-transcription polymerase chain reaction. We estimated DENV RNA prevalence and the number of DENV infections among blood donors. Results: A total of 4976 specimens were tested for DENV RNA, and 21 were TMA-reactive. The detection rate was 0.84 (95% confidence interval [CI], 0.15-4.73), 3.36 (95% CI, 1.31-8.60), and 6.19 (95% CI, 3.14-12.17) per 1000 donors in districts where the weekly dengue incidence was 5-50, 50-200, and 200 or more per 100 000 residents, respectively. Alanine aminotransferase screening only detected 4.4% of TMA-reactive donations. A total of 143 transfusion-transmitted DENV infections probably occurred during this outbreak, accounting for 9.2 in 10 000 dengue infections. Conclusions: Approximately 0.5%-1% of blood donations were DENV RNA positive in epidemic districts. The correlation of DENV RNA rates with dengue incidence may inform the design of effective control measures.enblood safety | dengue | transfusion-transmitted infection[SDGs]SDG3journal article10.1093/infdis/jiac014350990022-s2.0-85129997612https://api.elsevier.com/content/abstract/scopus_id/85129997612