Wong, Tzyy-YueTzyy-YueWongJuang, Wang-ChuanWang-ChuanJuangCHIA-TI TSAITseng, Ching-JiunnChing-JiunnTsengLee, Wen-HsienWen-HsienLeeSHENG-NAN CHANGCheng, Pei-WenPei-WenCheng2023-05-082023-05-082018-11-022077-0383https://scholars.lib.ntu.edu.tw/handle/123456789/630907The dynamics of a living body enables organs to experience mechanical stimulation at cellular level. The human cardiomyocytes cell line provides a source for simulating heart dynamics; however, a limited understanding of the mechanical stimulation effect on them has restricted potential applications. Here, we investigated the effect of mechanical stimulation on the cardiac function-associated protein expressions in human cardiomyocytes. Human cardiomyocyte cell line AC16 was subjected to different stresses: 5% mild and 25% aggressive, at 1 Hz for 24 h. The stretched cardiomyocytes showed down-regulated Piezo1, phosphorylated-Ak transforming serine473 (P-AKTS473), and phosphorylated-glycogen synthase kinase-3 beta serine9 P-GSK3βS9 compared to no stretch. In addition, the stretched cardiomyocytes showed increased low-density lipoprotein receptor-related protein 6 (LRP6), and phosphorylated-c-Jun N-terminal kinase threonine183/tyrosine185 (P-JNKT183/Y185). When Piezo inhibitor was added to the cells, the LRP6, and P-JNKT183/Y185 were further increased under 25%, but not 5%, suggesting that higher mechanical stress further activated the wingless integrated-(Wnt)-related signaling pathway when Piezo1 was inhibited. Supporting this idea, when Piezo1 was inhibited, the expression of phosphorylated-endothelial nitric oxide synthase serine1177 (P-eNOSS1177) and release of calcium ions were reduced under 25% compared to 5%. These studies demonstrate that cyclic mechanical stimulation affects cardiac function-associated protein expressions, and Piezo1 plays a role in the protein regulation.enPiezo1; cardiomyocytes; mechanical stimulation; stretchingjournal article10.3390/jcm7110410304002592-s2.0-85064485655WOS:000451311900033https://api.elsevier.com/content/abstract/scopus_id/85064485655