Kuo, C.-T.C.-T.KuoWang, J.-Y.J.-Y.WangLin, Y.-F.Y.-F.LinWo, A.M.A.M.WoChen, B.P.C.B.P.C.ChenANDREW WOH. Lee2020-04-282020-04-282017https://scholars.lib.ntu.edu.tw/handle/123456789/485849Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments. ? 2017 The Author(s).[SDGs]SDG3biocompatible coated material; dimeticone; bioassay; cell culture technique; coculture; human; multicellular spheroid; preclinical study; procedures; tumor cell line; Biological Assay; Cell Culture Techniques; Cell Line, Tumor; Coated Materials, Biocompatible; Coculture Techniques; Dimethylpolysiloxanes; Drug Evaluation, Preclinical; Humans; Spheroids, Cellularjournal article10.1038/s41598-017-04718-12-s2.0-85021651388https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021651388&doi=10.1038%2fs41598-017-04718-1&partnerID=40&md5=cd8ffde01f4490bc03a7f3c100b724cd