Sri-In C.SHIH-CHE WENGSHIN-HONG SHIAOTu W.-C.2020-06-302020-06-3020201932-6203https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085904531&doi=10.1371%2fjournal.pone.0233618&partnerID=40&md5=7e3489974693f002e718bc7274472818https://scholars.lib.ntu.edu.tw/handle/123456789/506705A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the artificial blood feeder versus mice as a live blood source. Significantly more engorged females Aedes aegypti fed on the artificial blood feeder than on mice. In addition, the artificial feeder could serve as a useful apparatus for oral infection via artificial blood meals, and for saliva collection in mosquitoes. Our method enabled us to collect saliva from multiple mosquitoes at once, providing sufficient infected saliva for determination of the virus titer by plaque assay analysis. Our artificial feeder has the advantage that it is simple, inexpensive, and efficient. ? 2020 Public Library of Science. All rights reserved.[SDGs]SDG3saliva protein; adult; Aedes aegypti; Aedes albopictus; animal cell; animal experiment; Article; cell culture; controlled study; dengue; Dengue virus 2; egg laying; female; mouse; mouth infection; nonhuman; protein analysis; saliva analysis; sanguivore; viral plaque assay; virus detection; virus load; Aedes; animal; animal food; dengue; Dengue virus; human; isolation and purification; mosquito vector; physiology; saliva; virology; Aedes; Animal Feed; Animals; Dengue; Dengue Virus; Female; Humans; Mice; Mosquito Vectors; Salivajournal article10.1371/journal.pone.0233618324699542-s2.0-85085904531