Tsai K.-C.MING-JIUM SHIEHYang S.-J.WEN-SHIANG CHEN2020-03-302020-03-3020089781420085075; 9781420085044https://www.scopus.com/inward/record.uri?eid=2-s2.0-52649172204&partnerID=40&md5=1555ebf340b8b2d2fa576b2f3afcfa82https://scholars.lib.ntu.edu.tw/handle/123456789/481488Photodynamic medicine is a novel approach for cancer detection and treatment via different photosensitizers and suitable light sources. The 5-Aminolevulinic acid (5-ALA) can be converted into protoporphyrin IX (PpIX) that can be utilized as a fluorescent probe for tumor detection. However, the fluorescent quantity of PpIX is sometimes too low for detection. In this study, 5-ALA was encapsulated in chitosan nanoparticles, and then pushed into HeLa cancer cells by ultrasound to increase the fluorescent quantity of PpIX. Chitosan nanoparticles loaded with 5-ALA (CNA) was prepared by ionic gelation method after mixing chitosan with 5-ALA.The size and zeta potential of CNA, measured by transmission electron microscope and zetasizer, was 102.4 nm and 25.0 mV, respectively, which were enough to prevent particles from aggregating. The loading efficiency of 5-ALA in CNA was up to 90%. The amounts of CNA uptaken by HeLa cells and the PpIX concentration in cells were significantly enhanced by ultrasound. Based on the above results, a novel photodynamic detection system might be designed to enhance the diagnostic accuracy of early cervical cancer.[SDGs]SDG3Acoustic waves; Amines; Cells; Chitin; Chitosan; Coagulation; Colloids; Commerce; Concentration (process); Exhibitions; Fluorescence; Gelation; Health; Light; Light emission; Light sources; Lighting; Luminescence; Mixing; Nanoparticles; Nanostructured materials; Nanostructures; Nanotechnology; Photosensitizers; Sensors; Technology; Transmission electron microscopy; Ultrasonics; Zeta potential; 5-Aminolevulinic acid; Cancer cells; Cancer detection; Cervical cancers; Chitosan nano-particles; Detection systems; Diagnostic accuracy; Fluorescent probing; HeLa cells; Ionic gelation method; Life sciences; Loading efficiency; Nanoparticle and ultrasound; Photodynamic medicine; Protoporphyrin IX; Trade shows; Transmission electron microscope; Tumor detection; Zeta potentials; Ultrasonic applicationsconference paper2-s2.0-52649172204