Yong-Shau ShienShyh-Renn ChouGUAN-TARN HUANG2023-01-132023-01-131996-06-01https://www.airitilibrary.com/Publication/alDetailedMesh?docid=02539179-199606-201210080001-201210080001-192-204https://scholars.lib.ntu.edu.tw/handle/123456789/627234The aim of this study was evaluate the possibility of primary culture hepatocytes separated from congenitally infected with duck hepatitis B virus (DHBV) ducklings as a tool for screening the drug with anti-hepadnaviral activity. Eleven extracts from four medicinal herbs with strong anti-DHBV endogenous DNA polymerase(DNAP) activity were employed in this study. Changes of the following items were carefully investigated: the hepatocyte morphology; the activities of lactate dehydrogenase and alanine aminotransferase in the culture media; the DHBV-DNA concentration in hepatocytes and in the culture media; and the intermediate products of DHBV-DNA in hepatocytes. Result revealed that this screening system had following excellent characters: not necessary to infect the cells after separation; easy to evaluate the toxicity and the ability to cross the cell membrane of the drugs; and easy to infer the mechanism of anti-DHBV replication of the drug. Therefore, this screening system could be an economic, time-saving, Semi-in vitro and semi-in vivo method to screen a huge number of drugs.zh鴨B型肝炎病毒鴨肝細胞培養抗親肝DNA病毒藥物DHBVDuck hepatocyte cultureAnti-hepadnaviral drugs[SDGs]SDG3journal article10.7009/JTCSVS.199606.0192