Hsiao, Yu ShengYu ShengHsiaoQuiñones, Edgar DanielEdgar DanielQuiñonesYen, Shih ChiehShih ChiehYenJIASHING YUFang, Ji TsengJi TsengFangChen, PeilinPeilinChenJuang, Ruey ShinRuey ShinJuang2023-06-092023-06-092023-01-0119448244https://scholars.lib.ntu.edu.tw/handle/123456789/632027Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis, thereby playing an essential role as both a viable alternative and valuable complement to conventional biochemical/pharmaceutical analysis in the field of diagnostics. Constant improvements are naturally sought to further improve the effective range and reliability of this technology. In this study, we developed poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes integrated into homemade ECIS cell-culture chamber slides for the simultaneous drug release and real-time biosensing of cancer cell viability under drug treatment. The CP comprised tailored PEDOT:PSS, poly(ethylene oxide) (PEO), and (3-glycidyloxypropyl)trimethoxysilane (GOPS) capable of encapsulating antitumor chemotherapeutic agents such as doxorubicin (DOX), docetaxel (DTX), and a DOX/DTX combination. This device can reliably monitor impedance signal changes correlated with cell viability on chips generated by cell adhesion onto a predetermined CP-based working electrode while simultaneously exhibiting excellent properties for both drug encapsulation and on-demand release from another CP-based counter electrode under electrical stimulation (ES) operation. Cyclic voltammetry curves and surface profile data of different CP-based coatings (without or with drugs) were used to analyze the changes in charge capacity and thickness, respectively, thereby further revealing the correlation between their drug-releasing performance under ES operation (determined using ultraviolet-visible (UV-vis) spectroscopy). Finally, antitumor drug screening tests (DOX, DTX, and DOX/DTX combination) were performed on MCF-7 and HeLa cells using our developed CP-based ECIS chip system to monitor the impedance signal changes and their related cell viability results.enconducting polymer (CP) | drug release | drug screening | electric cell-substrate impedance sensing (ECIS) | poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)[SDGs]SDG3journal article10.1021/acsami.3c02769371291062-s2.0-85159602676WOS:000984394500001https://api.elsevier.com/content/abstract/scopus_id/85159602676