方俊民臺灣大學：化學研究所周子琪Chou, Tzu-ChiTzu-ChiChou2007-11-262018-07-102007-11-262018-07-102004http://ntur.lib.ntu.edu.tw//handle/246246/51783本篇論文主要是利用磁性奈米粒子來與生物分子結合，希望藉由生物分子的專一辨識性，加上利用磁性來達到分離純化的效果。 如下圖所示，我們將其分為三個部份，一是四氧化三鐵磁性奈米粒子，其上包覆有聚丙烯酸來避免四氧化三鐵的氧化且使奈米粒子能均勻的溶於水中，並藉由羧酸這個官能基，將連接鏈接到奈米粒子上；連接鏈的設計方面，由於需考慮水溶性來配合生物分子，故採用水溶性佳的乙二醇為架構；在連接鏈末端，留了一個雙硫吡啶來與生物分子上的硫醇做交換，而將生物分子以雙硫鍵接上磁性奈米粒子，交換後所得到的2-吡啶硫酮也可利用其含UV吸收的特性，來反定量接上生物分子的多寡；這裡使用的生物分子是禽流感H6型的病毒與抗體，並利用血球凝集試驗來證明病毒存在與否。In this thesis, we describe the preparation of magnetic nanoparticles linked with biomolecules, such as antibody, for specific recognition and easy separation of the target receptors, such as virus, and purified them. As shown in the figure, three parts are connected. The first part is ferrite magnetic nanoparticles (NPs) which were covered with poly (acrylic acid) to prevent oxidation and to increase solubility in water. These acid groups are connected with the ethylene glycol type linkers with good water solubility. At the end of the linker, there is a dithiolpyridine moiety which can form a disulfide bond with the thiol groups on biomolecules. In the mean time of this sulfide exchange, 2-thiolpyridone is released and detected by UV absorption. This method is used to quantify the amount of attacked biomolecules. The biomolecules in my study include H6 avian influenza virus (AI virus) and its antibody. In the first approach, the virus is directly attacked on NPs. Alternatively, the virus-specific antibody is first attacked to the NPs, and then used to detect AI virus. Hemagglutination test is used to detect if there’s virus or not in the test sample.目錄…………………………………………………………………I 圖表索引……………………………………………………………III 化學符號縮寫………………………………………………………V 中文摘要……………………………………………………………VI 英文摘要……………………………………………………………VII 第一章 緒論………………………………………………………1 1.1前言……………………………………………………………1 1.2多價作用力……………………………………………………2 1.3生物分子與奈米粒子的結合…………………………………3 1.4金奈米粒子……………………………………………………4 1.5量子點…………………………………………………………6 1.6磁性奈米粒子…………………………………………………8 1.7動物流行性感冒病毒………………………………………15 1.8研究目標設計………………………………………………18 第二章 結果與論……………………………………………20 2.1 磁性奈米粒子之合成………………………………………21 2.2 磁性奈米粒子之表面修飾…………………………………27 2.2.1三氮唑……………………………………………………27 2.2.2 疊氮………………………………………………………29 2.2.3溴化乙醯溴………………………………………………31 2.2.4雙硫二吡啶………………………………………………32 2.3奈米粒子與生物分子的連結和測試………………………35 2.3.1未修飾連接鏈的奈米粒子與生物分子的連結…………35 2.3.2 修飾有連接鏈的奈米粒子與生物分子的連結…………41 2.4 結論…………………………………………………………44 第三章 其他研究部分…………………………………………45 3.1 醣類分子的合成……………………………………………45 3.2 連接鏈的合成………………………………………………46 3.3 醣類分子與連接鏈的結合…………………………………47 3.4 金奈米粒子的合成…………………………………………48 3.5 奈米粒子的表面修飾與測試………………………………49 3.6 結論…………………………………………………………50 第四章 實驗部分………………………………………………51 參考文獻…………………………………………………………74 部分光譜資料……………………………………………………795529530 bytesapplication/pdfen-US磁性奈米生物辨識nanomagneticbiomolecule recognitionthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/51783/1/ntu-93-R91223042-1.pdf