2014-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662989摘要：植物交互保護 (cross-protection) 是藉由植物預先感染與強系病毒親緣相近的弱系病毒，進而導致植物產生抵抗強系病毒的抗性反應。在先期國科會計畫中，我們已經建立蕪菁嵌紋病毒 (Turnip mosaic virus, TuMV) 的弱系病毒株 - Tu-GK 與阿拉伯芥 (Arabidopsis thaliana) 為主體的交互保護研究平台。先前的結果顯示，植物交互保護是一種多元性抗病反應 (multiple immunities)，其中包含了後轉錄基因靜默 (post-transcriptional gene silencing, PTGS) 作用、蛋白誘導的抗病機制 (protein-mediated resistance) 及植物內生抗性 (innate immunity) 之保護作用。本研究將探討交互保護作用機制中多元抗病性之反應路徑，其主要內容有以下四點：(1) 評估 Tu-GK 誘導之植物內生抗性對不同種及不同屬病毒的抗病能力；(2) 搜集不同抗病路徑的阿拉伯芥轉基因株與突變株；(3) 評估 Tu-GK 誘導之植物內生性抗病反應，在不同抗病路徑的阿拉伯芥轉基因株與突變株中之保護能力；(4) 利用高通量定序分析 TuMV 所感染之獨立免疫反應植株，用以探討不同抗病反應之能力。另一方面，則以病毒累積時程 (time-course pattern) 及高通量定序分析其轉錄體 (transcriptomes) 、病毒小分子核酸 (virus-directed short interfering RNA, vsiRNA) 及降解體 (degradome) 變化，用以探討寄主與病毒間的交互作用，進而深入了解由交互保護作用所引起的多元性抗病反應。<br> Abstract: We developed a Turnip mosaic virus (TuMV) mild strain (Tu-GK) mediated cross-protection system in Arabidopsis thaliana plant in the previous project of National Science Council (NSC). Our previous study indicated that the multiple immunities that include a post-transcriptional gene silencing (PTGS) pathway, protein-mediated resistance, and host innate immunities confer crucial roles in Tu-GK-mediated cross-protection. In this proposal, we plan to further explore the multiple immunity pathways in cross-protection. Four specific goals will be highlighted in this study. (1) The evaluation efficiency of Tu-GK-mediated cross-protection between different species or genera of viruses. (2) Collection of mutants or generation of transgenic Arabidopsis materials in various immunity pathways. (3) An evaluation of the efficiency of Tu-GK mediated cross-protection in various mutants and transgenic plants. (4) The collection of high-throughput deep sequence profiles from TuMV infected samples. The primary purpose is to individually study virus resistance efficiency in various immunities. In addition, the virus accumulation time-course patterns and high-throughput deep sequence profiles in viral small RNAs, transcriptomes, and degradomes provide quality insights into host-virus interaction, enabling an understanding of the multiple immunities provided by cross-protection.交互保護多元抗性反應後轉錄時期基因靜默機制蛋白媒介抗性內生性免疫反應次世代基因定序Cross-protectionmultiple immunitiespost-transcriptional gene silencingprotein-mediated resistanceinnate immunitynext-generation sequence.