2009-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/710612摘要：隨著分子生物學的快速發展，微生物檢驗儀器的精密程度以及方便程度上的需求大為激增。然而現階段的商用化規格遭受到巨大的挑戰：體積龐大而攜帶不易、接觸式檢測導致儀器易受污染。本計畫提出一新式的可攜式生物檢測系統，同時結合射頻微機電技術、奈米顆粒與微流體傳輸系統等跨領域的整合技術，以期克服這些困境。本系統的主要原理是利用射頻共面波導的電路，並且在電路結構中植入奈米材料的共軛高分子，當病原體的核酸序列進入該共軛結構之後，會連接奈米金顆粒，而目標核酸的濃度變化會改變射頻共面波導的電磁行為，因此可達成定量檢測核酸的目的。本檢測系統將在三年內完成。第一年利用現有製程技術，設計並實現一共面波導結構的高頻生物感測器；同時定義出感測器的靈敏度以及再現性。第二年則是應用多層奈米金以及奈米磁性顆粒的生物條碼核酸放大技術將靈敏度加以提升。第三年的重點則是整合微流體傳輸系統以達到輕薄短小的目標。 本計劃預期能達成的目標為 (1) 實現UHF頻段的高頻共面波導元件。 (2) 驗証高頻生物感測器的定量分析能力，樣本最低檢測濃度在1fM。 (3) 多層奈米金以及生物條碼核酸放大後，信號解析度應為放大前的100倍。 (4) 高頻生物感測器的晶片大小在 6mm × 6mm之內。 (5) 樣本可容納空間容量在100μL以上。 (6) 整合微流體晶片成一無線生物分子檢測系統。 <br> Abstract: As the fast development of the molecular biology, it becomes more demanding for a rapid and accurate instrument that can used to detect biomolecules. Nevertheless, there are tremendous challenges for such a commercial instrument to face such as miniaturization of instrument and contamination of detected sample on the instrument. Therefore, a novel portable and miniature bio-detection system is proposed in this project. With highly integrations of RF MEMS technology, nanoparticles and micro fluidic devices, the proposed system is expected to be capable of overcoming those obstacles. The major principles applied to this system are described as follows. A biochip with a coplanar waveguide circuit was established on a glass substrate by using a MEMS technology. Specific hybridization among ssDNA is used to establish bio-nanoparticle-conjugate nanostructures on the detection surface of biochip. When the bio-nanoparticle-conjugate nanostructures are established on the detection surface of the coplanar waveguide circuits, the electromagnetic behaviors of the circuits are changed due to the existence of the bio-nanoparticle-conjugate nanostructures. In addition, the electromagnetic behavior of circuit depends on how dense of nanostructure can be established on the surface, which is affected by the concentration of target DNA strand. Therefore, the system can be used to quantify the concentration of target DNA in the tested sample. This project is planed to be completed within 3 years. The mission of the first year is to design and implement a RF biosensor consisting of coplanar waveguide circuits; meanwhile to define the sensitivity and reproducibility. The mission of the second year is to apply the gold nanoparticles and magnetic nanoparticles to enhance the sensitivity of the current devices. The aim of the third year is to integrate the micofluidic system to be more compact. The major outcomes that can be achieved by this project are: (1) The implementation of a CPW device that is operated at a UHF band. (2) Verification of the quantification ability of our developed biochip, the detection limitation could be as low as 1fM. (3) The resolution of the amplified sensing signals should be 100 fold of those before amplification. (4) The chip size is within the area of 6mm × 6mm. (5) The volume of the sample container is larger than 100μL. (6) A integrated system with microfluidic devices has the capability for wireless transmission of detection signals of target biomolecules in the tested sample.射頻生物感測器DNA微流道Radio-frequencybiosensorDNAmicro-channel