Lachman H.M.Fann C.S.J.Bartzis M.Evgrafov O.V.Rosenthal R.N.Nunes E.V.Miner C.Santana M.Gaffney J.Riddick A.CHIA-LIN HSUKnowles J.A.2020-12-242020-12-2420070964-6906https://www.scopus.com/inward/record.uri?eid=2-s2.0-34447341994&doi=10.1093%2fhmg%2fddm081&partnerID=40&md5=3af60f11e5caae10a5242f264e124197https://scholars.lib.ntu.edu.tw/handle/123456789/531140The genetic predisposition to addiction to opioids and other substances is transmitted as a complex genetic trait, which investigators are attempting to characterize using genetic linkage and association. We now report a high-density genome-wide linkage study of opioid dependence. We ascertained 305 DSM-IV opioid dependent affected sibling pairs from an ethnically mixed population of methadone maintained subjects and genotyped their DNA using Affymetrix 10K v2 arrays. Analysis with MERLIN identified a region on chromosome 14q with a non-parametric lod (NPL) of 3.30. Secondary analyses indicated that this locus was relatively specific to the self-identified Puerto Rican subset, as the NPL increased from 3.30 to 5.00 (NPL(Caucasian) = 0.05 and NPL(African Amer.) = 0.15). The 14q peak encompasses the NRXN3 gene (neurexin 3), which was previously identified as a potential candidate gene for addiction. Secondary analyses also identified several regions with gender-specific NPL scores greater than 2.00. The most significant was a peak on (10q) that increased from 0.90 to 3.22 when only males were considered (NPL(female) = 0.05). Our linkage data suggest specific chromosomal loci for future fine-mapping genetic analysis and support the hypothesis that ethnic and gender specific genes underlie addiction susceptibility.[SDGs]SDG3journal article10.1093/hmg/ddm081174091922-s2.0-34447341994