Lee, C.-W.C.-W.LeeChen, M.J.M.J.ChenCheng, J.-Y.J.-Y.ChengWei, P.-K.P.-K.WeiMIIN-JANG CHEN2020-05-122020-05-122009https://scholars.lib.ntu.edu.tw/handle/123456789/491790The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times. ? 2009 Society of Photo-Optical Instrumentation Engineers.image reconstruction; microscopy; scattering; surface plasmons[SDGs]SDG3Cell morphology; Cytochalasin D; Dark-field; Electrostatic attractive forces; Focal Plane; Gold Nanoparticles; l-Lysine; Living cell; Lung cancer cells; Morphologic changes; Morphological images; Morphological study; Optical sections; Scattering intensity; surface plasmons; Amino acids; Drug interactions; Gold; Image reconstruction; Nanoparticles; Three dimensional; Bioinformatics; cytochalasin D; gold; metal nanoparticle; tumor necrosis factor alpha; algorithm; article; cell; cell culture; cell shape; chemistry; cytology; drug effect; HeLa cell; human; image processing; lung non small cell cancer; lung tumor; metabolism; methodology; microscopy; pathology; surface plasmon resonance; tissue distribution; tumor cell culture; Algorithms; Carcinoma, Non-Small-Cell Lung; Cell Shape; Cells; Cells, Cultured; Cytochalasin D; Gold; Hela Cells; Humans; Image Processing, Computer-Assisted; Lung Neoplasms; Metal Nanoparticles; Microscopy; Surface Plasmon Resonance; Tissue Distribution; Tumor Cells, Cultured; Tumor Necrosis Factor-alphajournal article10.1117/1.31473902-s2.0-70349257422https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349257422&doi=10.1117%2f1.3147390&partnerID=40&md5=6ddf7808363bfabba19898344d17b729