國?臺灣大學生態學及演化生物學研究所與生命科學系Institute of Ecology and Evolutionary Biology, Department of Life Science, National Taiwan University王俊能(Chun Neng Wang)陳彥君(Yan-Jun Chen)張詠嬋(Yung-Chan Chang)吳俊賢(Chun-Hsien Wu)2017-09-122018-07-062017-09-122018-07-062008-12http://ntur.lib.ntu.edu.tw//handle/246246/283441http://ntur.lib.ntu.edu.tw/bitstream/246246/283441/1/5304_200812_5.pdfTissue specific RNA in-situ hybridization is a critical technique required for botanists to examine exact gene expression domain within plant tissues. This powerful technique provides a way to examine the spatial and temporal patterns of gene expression of plants and animals in developmental stages content and at tissue cellular level. However, this technique has been largely hampered by difficulties of obtaining reliable protocols for non-model organism. Here we offer our lab experiences on how to optimize in-situ hybridization process among various plant species. This step-by-step guide however should not be regarded as the only solution to resolve the difficulties of RNA tissue specific RNA in-situ hybridization. Instead, the key optimization steps including tissue fixation time, proteinase/pronase treatment, probe length/content and hybridization time are priority issues to be tested first. We further summarized some recent advance of literature on alternative ways to conduct in-situ hybridization process.8153752 bytesapplication/pdfdigoxigenin (DIG) – labeled RNA probeshistone genein-situ hybridizationAfrican violetTitanotrichum oldhamii毛地黃素非放射性雜交探針組織蛋白基因原位雜交技術非洲堇俄氏草[SDGs]SDG15journal article10.6165/tai.2008.53(4).383http://ntur.lib.ntu.edu.tw/bitstream/246246/283441/1/5304_200812_5.pdf