Department of Internal Medicine, National Taiwan University Hospital鄭之勛2006-07-262018-07-112006-07-262018-07-112005http://ntur.lib.ntu.edu.tw//handle/246246/23710Rationale: Mechanical ventilation may be responsible not only for worsening the underlying lung injury, but may also lead to the development of new lung injury, i.e., ventilator-induced lung injury (VILI) as well as systemic inflammatory response syndrome and multiple organ failure. Objectives: To study the role of macrophage inflammatory protein-2 (MIP-2), a functional equivalent of interleukin-8 of the chemokine group, in the pathogenesis of VILI, and to investigate the renin-angiotensin system in the involvement of VILI and possible of regulation of MIP-2 expression in VILI. Methods: Sprague-Dawley rats were mechanically ventilated for four hours with either low (7 ml/kg) or high (40 ml/kg) tidal volumes. Histologic studies, measurements of the myeloperoxidase activity and nuclear factor-κ B activity, quantification of the mRNA as well as protein levels of MIP-2 in the lungs, and assessment of the expression after treatment with captopril, and angiotensin-converting enzyme inhibitor, were performed. Main Results: In the high-volume group, lung tissue neutrophil infiltration, activity of nuclear factor -κ B, tumor-necrosis factor-α , and macrophage inflammatory protein-2 were significantly increased, and survival was significantly reduced. Treatment with captopril attenuated the lung neutrophil infiltration, tumor-necrosis factor-α , macrophage inflammatory protein-2, and nuclear factor-κ B activity in the high-volume group, with significant survival benefit. Conclusions: Injurious mechanical ventilation is associated with significantly increased expression of macrophage inflammatory protein-2 in rat lungs, with prominently increased neutrophil infiltration. Treatment with angiotensin-converting enzyme inhibitor can attenuate MIP-2 expression and ventilator-induced lung injury in the animal model.application/pdf179781 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科Ventilator-induced lung injurymacrophage-inflammatory protein-2angiotensin-converting enzymereporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/23710/1/932314B002223.pdf