Tseng L.-PChiou C.-JDeng M.-CLin M.-HPan R.-NYI-YOU HUANGLiu D.-Z.2020-02-272020-02-2720091552-4973https://scholars.lib.ntu.edu.tw/handle/123456789/465147We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal-Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination-inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme-linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 μmol), cholesterol (Chol) (15 μmol), and EPC-liposomes (EPC-Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 μmol), stearylamine (SA) (15 μmol), Chol (15 μmol), and SA-liposomes (SA-Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 μmol), dipalmitoylphosphatidylserine (15 μmol), Chol (15 μmol), and phosphatidylserine-liposomes (PS-Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPCLip is very useful in enhancing antibody production at the mucosal site and in serum. ? 2009 Wiley Periodicals, Inc.Adjuvant; Intranasal delivery; Liposomal vaccine; Newcastle disease virus[SDGs]SDG3Adjuvant; Antibody production; Antibody titers; Antigen delivery; Enzyme linked immunosorbent assay; Humoral immune response; Humoral immunity; Immune response; Immunogenicity; Intranasal; Intranasal delivery; Liposomal vaccine; Phosphatidylcholine; Phosphatidylserine; Phospholipid liposomes; Antibodies; Antigens; Body fluids; Dyes; Enzyme inhibition; Immunology; Phospholipids; Simulated annealing; Vaccines; Liposomes; cholesterol; immunoglobulin A; immunoglobulin G; liposome; Newcastle disease vaccine; newcastle disease virus liposome; phosphatidylcholine; phospholipid; unclassified drug; virus antigen; animal experiment; antibody production; antibody titer; article; chicken; controlled study; enzyme linked immunosorbent assay; hemagglutination inhibition; humoral immunity; immune response; immunization; immunogenicity; Newcastle disease paramyxovirus; nonhuman; Animals; Antibodies, Viral; Chickens; Drug Carriers; Drug Compounding; Enzyme-Linked Immunosorbent Assay; Excipients; Hemagglutination Inhibition Tests; Immunity, Humoral; Immunization; Immunoglobulin G; Liposomes; Newcastle disease virus; Particle Size; Phospholipids; Trachea; Viral Vaccinesjournal article10.1002/jbm.b.31437195828532-s2.0-71649100288