CHIN-TIN CHENHuang C.-CRUEY-JIEN CHENLin Y.-HChiang H.HWang C.-YLee Y.-SSONG-NAN CHOW2020-02-192020-02-1919970929-6646https://scholars.lib.ntu.edu.tw/handle/123456789/460820In Taiwan, cervical cancer is the leading malignancy among women. For the early detection of cervical neoplasia, light-induced fluorescence spectroscopy was conducted ex vivo to assess the ability of this technique to differentiate cervical neoplastic tissue (20 samples) from normal or inflammatory cervical tissue (37 samples) at an excitation wavelength of 280 nm. The principal fluorescent peaks occurred within ±5 nm of 330 nm and 470 nm emission. At 330 nm emission, the spectrum of the normal or inflammatory tissue was significantly stronger than that of the neoplastic tissue after area normalization. However, at 470 nm emission, the spectrum of the normal or inflammatory tissue was significantly weaker than that of the neoplastic tissue. A diagnostic algorithm based on the ratio of relative intensities of 330 nm to 470 nm emission within the ±5 nm peak area of each sample was calculated and paired. The ratios showed that histologically neoplastic lesions could be distinguished from inflammatory samples using a 280-nm-excitation wavelength with a sensitivity, specificity and positive predictive value of 94%, 82% and 73%, respectively. The average ratio of malignant or dysplastic cervical samples was significantly greater than that of the inflammatory samples (p < 0.001). Our ex vivo study indicated that light-induced fluorescence spectroscopy may be useful in differentiating malignant or premalignant from normal or inflammatory cervical tissue.cervical neoplasia; fluorescence spectroscopy[SDGs]SDG3article; cancer diagnosis; clinical article; clinical trial; controlled clinical trial; controlled study; diagnostic value; differential diagnosis; female; fluorescence microscopy; human; human tissue; uterine cervix cancer; Algorithms; Diagnosis, Differential; Female; Humans; Sensitivity and Specificity; Spectrometry, Fluorescence; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms; Uterine Cervicitisjournal article91365102-s2.0-0031011075