CHENG-PING WANGPEI-JEN LOULo F.-Y.MING-JIUM SHIEH2020-11-032020-11-0320140929-6646https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895870289&doi=10.1016%2fj.jfma.2012.05.006&partnerID=40&md5=9463e461f06bd99c41ae6179125962d7https://scholars.lib.ntu.edu.tw/handle/123456789/520308Background/Purpose: Different photosensitizer-mediated photodynamic therapy (PDT) has different intracellular cytotoxic cascades. Previous reports showed that 5-aminolevulinic acid (5-ALA)-mediated PDT suppressed the migration and invasion of head and neck cancer cells.Unlike from 5-ALA, which mainly targets the mitochondria of cells, meta-tetrahydroxyphenyl chlorin (m-THPC) mainly accumulates in the endoplasmic reticulum and Golgi complex. Does m-THPC PDT inhibit the migration and invasion of cancer cells? Methods: The effect of m-THPC PDT with a sublethal dose sufficient to kill around 20% of cells on the migration and invasion of a nasopharyngeal carcinoma KJ-1 cell line was studied by wound healing and Matrigel invasion assays. Results: In the wound healing assay, the migration distance of KJ-1 cells decreased significantly from 0.71±0.02mm in the control cells to 0.31±0.03mm in the PDT-treated cells 24 hours after light treatment (p<0.05) and the migration distance also decreased significantly from 1.02±0.07mm in the control cells to 0.32±0.04mm in the PDT-treated cells 48 hours after treatment (p<0.05). In the Matrigel invasion assay, the number of the invaded KJ-1 cells in PDT treated group was also statistically significantly less than those without PDT treatment (p<0.05). Conclusion: This study demonstrates that a sublethal dose of m-THPC PDT inhibits the migration and invasion of nasopharyngeal carcinoma cells invitro. ? 2012.[SDGs]SDG3matrigel; tetrakis(3 hydroxyphenyl)chlorin; article; cancer cell; cancer cell culture; cancer inhibition; cell death; cell invasion; cell migration; cell viability; concentration response; controlled study; cytotoxicity; endoplasmic reticulum; Golgi complex; human; human cell; in vitro study; metastasis; metastasis inhibition; migration inhibition; MTT assay; nasopharynx carcinoma; photodynamic therapy; tumor invasion; undifferentiated carcinoma; Foscan; head and neck cancer; metastasis; photodynamic therapy; photosensitizer; Antineoplastic Agents; Cell Line, Tumor; Cell Migration Assays; Cell Movement; Cell Survival; Humans; Mesoporphyrins; Nasopharyngeal Neoplasms; Neoplasm Invasiveness; Photochemotherapy; Photosensitizing Agents; Wound Healingjournal article10.1016/j.jfma.2012.05.006246300352-s2.0-84895870289