2008-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684915摘要：茄科青枯病係複雜且重要土壤傳播性病害，基於抗病育種之困難性、抗病策略需求之迫切性及植物土壤傳播系統性病害抗病機制重要訊息嚴重缺乏等考量，本計畫針對此病害，同時利用生物晶片及病毒誘發基因靜默(VIGS)兩種可互補的研究策略，研發在作物保護及其他農業生技上具應用性之抗病防禦基因。目前已完成多項具新穎性及應用潛力的研究成果： (1)利用番茄抗青枯病品系，已建構subtractive cDNA libraries及cDNA microarrays等重要研發資源，這些研究資源將可應用於作物重要病害之抗病機制的分析與抗病品系之快速篩選。 (2)已改良病毒誘發基因靜默載體，並建立適合茄科作物土壤傳播性病害研究之高效VIGS系統及技術平台，不但可適用於進行快速大量測試與篩選青枯病防禦基因之用，此系統及所得know-how亦可應用於其他生物/非生物逆境及各項農作物研究課題之基因體研究。 (3)利用此高效VIGS系統，已陸續大量分析選自特製番茄array試驗之病菌誘導性基因群以及茉莉酸/乙烯/水楊酸訊息傳遞路徑相關之特定防禦基因群，在抗青枯病機制中扮演的角色，並直接大量篩選番茄青枯病抗病的關鍵防禦基因，以深入解析番茄抗青枯病之抗病機制及訊息傳遞網絡。經VIGS試驗後，目前已陸續找到在青枯病抗病機制扮演重要角色的病害防禦基因，新年度的工作內容將針對其中數個新穎病害防禦基因，進一步深入地應用各種分生、生物資訊及基因轉殖研究策略，探討它們在抗/感病反應中可能扮演的角色，並研發在作物保護及其他農業生技上之應用性。本計畫預期所得之抗病防禦機制訊息與防禦基因群，將可應用於作物抗病育種（包括利用基因轉殖策略或在molecular mapping策略中當作重要markers）及其他病害防治策略。預計計畫產出包括技術平台與防禦基因之智慧財產權及各種衍生”know-hows”、論文/報告發表及人才培訓等。<br> Abstract: Transforming defense-related genes to induce plant resistance has become a common approach for disease management considering efficacy, efficiency, and reduced pesticide use. It is desired that the induced resistance should not only be durable and with broad spectrum, but also without deteriorating effect on plants. Thus, comprehensive information on genes involved in plant defense and resistance response would be highly desirable and necessary. We choose bacterial wilt of Solanaceaous crops as the case disease in this proposal as it is one of the most complex and serious crop diseases worldwide, and breeding for durable resistance for this disease is important and urgent. In addition, information on plant defense response to systemic infection of soilborne pathogens is very limited. We therefore intend to identify plant defense/inducible genes involved in tomato response to the infection of Ralstonia solanacerum, the causing agent of bacterial wilt. The approaches to be employed for the studies proposed herein include differential cDNA microarray hybridization and virus-induced gene silencing (VIGS). Furthermore, by combining various molecular and transgenic technologies, we are studying role(s) of selected defense/inducible genes in tomato disease-defense, as well as seek for potential applications of these genes for crop protection/improvement. Through these studies, we hope to identify a group of agronomically important and useful genes that may benefit our future work on tomato or relevant crop improvement. The projected outputs of these studies include platform technology development, patents and intellectual properties, publications and personnel training. Therefore, we expect this could benefit both fundamental and applied agricultural research.茄科作物青枯病防禦基因基因靜默DNA晶片Solanaceousbacterial wiltdisease defensevirus-induced gene silencingcDNA microarray