Hsiao, H.-Y.H.-Y.HsiaoCheng, Tzong-JihTzong-JihChengYang, G.-M.G.-M.YangHuang, I.-J.I.-J.HuangRICHIE CHEN2018-09-102018-09-102008http://www.scopus.com/inward/record.url?eid=2-s2.0-41149163216&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/338223A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60°C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 μ i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mM SDS in 10 mM borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 μ/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant. Copyright ? 2007 John Wiley & Sons, Ltd.application/pdf175570 bytesapplication/pdf9-methoxycamptothecin; Hydrophobic compounds; MEKC; Organic modifier; Sample pretreatment[SDGs]SDG3antineoplastic agent; boric acid; camptothecin; dimethyl sulfoxide; dodecyl sulfate sodium; Nothapodytes foetida extract; plant extract; unclassified drug; article; capillary electrophoresis; capillary zone electrophoresis; controlled study; drug determination; drug isolation; drug solubility; drug structure; hydrophobicity; linear system; medicinal plant; micellar electrokinetic chromatography; nonhuman; Nothapodytes foetida; pH measurement; quantitative analysis; temperature measurement; ultraviolet spectrophotometry; Angiosperms; Camptothecin; Dimethyl Sulfoxide; Electrophoresis, Capillary; Methanol; Molecular Structure; Plant Extracts; Solubility; Spectrophotometry, Ultraviolet; Nothapodytes foetidajournal article10.1002/pca.1026