2006-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682999摘要：5’ 端蓋頭結構的去除是信息核糖核酸裂解的一重要步驟。在真核細胞中演 化保守的兩個去蓋頭酵素1 與2 蛋白質以總酵素(holoenzyme)的方式一起作 用。去蓋頭酵素2 是總酵素中具有去蓋頭催化功能的蛋白次體，而以一至今未 明瞭的機制，去蓋頭酵素1 則是促進去蓋頭活性的結合蛋白因子。 吾人先前指出在發育功能上，果蠅去蓋頭酵素1，dDcp1，是osk mRNA 坐 落於卵細胞後端與其裂解所需要的重要成員(附件1). 至今果蠅去蓋頭酵素2， dDcp2 在發育過程中的角色依舊未明。在我們的初步實驗中，dDcp2 突變也呈現 出典型的胚胎後端突變性狀。這一計畫將以釐清果蠅去蓋頭酵素2，dDcp2，是 否也是osk mRNP 複合體中的成員為主要目標。 我們正進行分析已經搜尋到的新dDcp2 基因型式( allele)，包括null 與弱 P 嵌入突變；也將利用不同基因體序列片段以檢驗互補測試並確認由dDcp2 基因 <br> Abstract: The cleavage of the 5’ cap structure represents a critical step in mRNA degradation turnover. The two eukaryotic conserved decapping proteins, Dcp1 and Dcp2, function together as a holoenzyme. Dcp2 has been found to be the catalytic subunit of the decapping holoenzyme and Dcp1 primarily functions to enhance the decapping activity of Dcp2 by a currently unresolved mechanism. We have previously shown that Drosophila decapping protein 1, dDcp1, is essential for osk mRNA posterior deposition and degradation (Appendix 1). Up to date, the roles of dDcp2 during developmental processes are still unknown. In our preliminary test, mutation of dDcp2 also displays a typical posterior group embryonic phenotype. This project is aimed to clarify whether dDcp2 is also a novel component of osk mRNP complex. We are on the process in analyzing dDcp2 alleles recovered, including those null and weak P insertions. Complementation tests using different genomic fragments will be performed to confirm the mutant phenotype caused by dDcp2. In addition to the two peptide antibody available, a repetitive epitode antibody will be produced to reconfirm the protein expression pattern of dDcp2. The role of its developmental roles in oogenesis will be examined, particularly its function in osk mRNP complex formation. In this field, we first reported that dDcp1 acts for osk mRNA posterior localization. Since dDcp1 and dDcp2 form a bipartite protein complex in mRNA degradation system, this study on dDcp2 is expected to have a strong impact in cell biology and RNA related studies. This grant project is designed to further elucidate the machinery behind and to maintain the lead in this research area.果蠅去蓋頭酵素1果蠅去蓋頭酵素2核糖核酸復合體卵發育胚胎發育protein 1Drosophila deDrosophila decapping capping protein 2oskar mRNPoogenesisembryogenesis果蠅去頭蓋蛋白質的發育遺傳