Lin, J.H.J.H.LinWeng, C.N.C.N.WengLiao, C.W.C.W.LiaoYeh, K.S.K.S.YehPan, M.J.M.J.Pan2018-09-102018-09-102003http://www.scopus.com/inward/record.url?eid=2-s2.0-0037981504&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/301362The efficacy of Mycoplasma hyopneumoniae oral vaccine was investigated in microsphere dosage form. A co-spray drying process was used to apply an encapsulating material, Eudragit L30 D-55, to microspheres containing Mycoplasma hyopneumoniae antigens. The microspheres were generally effective (>93%) with protein release at pH 7.4, but almost none were released at pH 1.2, for 3 hr in an in vitro dissolution test. An SPF-swine model was used to evaluate the effectiveness of the microspheres as an oral vaccine, and the related immune responses. The serum's systemic IgG against M. hyopneumoniae was evoked by ELISA analysis, after a 2nd immunization of all pigs. The vaccinated groups' mean lesion score was significantly lower after the Mycoplasma hyopneumoniae challenge than that of the nonvaccinated/challenged groups (P<0.05). This study strongly suggests that the oral microspheres vaccine prepared by a co-spray drying method can provide effective protection against M. hyopneumoniae infection in pigs.Co-spray drying; Microsphere; Mycoplasma hyopneumoniae; Oral vaccine[SDGs]SDG3Mycoplasma hyopneumoniae; Mycoplasmatales; Sus scrofa; bacterial vaccine; bacterium antibody; microsphere; animal; animal disease; article; bacterial infection; blood; clinical trial; drug dosage form; drug formulation; immunology; lung; microbiology; Mycoplasma; oral drug administration; pathology; swine; swine disease; time; vaccination; Administration, Oral; Animals; Antibodies, Bacterial; Bacterial Vaccines; Dosage Forms; Drug Compounding; Lung; Microspheres; Mycoplasma; Mycoplasma Infections; Swine; Swine Diseases; Time Factors; Vaccinationjournal article10.1292/jvms.65.69