Fish oil enriched 6)-3 fatty acids ameliorates protein synthesis/degradation imbalance, inflammation, and wasting in muscles of diet-induced obese rats

SHING-HWA LIUChen, Yu-ChingYu-ChingChenTzeng, Huei-PingHuei-PingTzengChiang, Meng-TsanMeng-TsanChiang2023-02-212023-02-212021-121756-4646https://scholars.lib.ntu.edu.tw/handle/123456789/628606We investigated the effects of long-term supplementation of omega-3 fatty acids-enriched fish oil (FO) on preserving muscle mass. We found that dietary supplementation with 5% FO has a direct impact on preventing obesity and skeletal muscle loss in rats fed a high-fat diet for 16 weeks. The increased protein expression for ubiquitin ligases atrogin-1 and muscle RING-finger protein 1 (MuRF1) and the autophagy-related protein, PTEN-induced putative kinase 1 (PINK1) in muscles were both abrogated by FO. The AKT and mammalian target of rapamycin (mTOR) phosphorylation and the AMP‐activated protein kinase (AMPK)/peroxisome proliferator‐activated receptor γ coactivator 1α (PGC‐1α) signaling activation in muscles were up-regulated by FO. FO supplementation effectively reversed the increased plasma TNF-α levels and NF‐κB activation in muscles. These results suggest that FO supplementation prevents skeletal muscle wasting under obesity through activating AKT/mTOR and AMPK/PGC-1α signals and blocking NF-κB-mediated inflammatory pathway to improve the balance between protein synthesis and degradation.enFish oil; MUSCLE wasting; Protein synthesis; degradation; Inflammation; E3 UBIQUITIN-LIGASES; SKELETAL-MUSCLE; EICOSAPENTAENOIC ACID; INSULIN-RESISTANCE; ADIPOSE-TISSUE; FIBER TYPE; ATROPHY; MASS; APOPTOSIS; PATHWAY[SDGs]SDG3[SDGs]SDG14Fish oil enriched 6)-3 fatty acids ameliorates protein synthesis/degradation imbalance, inflammation, and wasting in muscles of diet-induced obese ratsjournal article10.1016/j.jff.2021.1047552-s2.0-85114909958WOS:000701795500008https://api.elsevier.com/content/abstract/scopus_id/85114909958