Liu, Chien-LiangChien-LiangLiuJUI-HUNG YEN2018-09-102018-09-102016-01-01http://www.scopus.com/inward/record.url?eid=2-s2.0-84955452347&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/396068The bacterial strain KR-12 was isolated from river sediment in northeast Taiwan. 16S rRNA gene sequencing revealed that it belongs to the genus Shewanella. The strain can accumulate lead (Pb) and form Pb nanoparticles (PbNPs) on exposure to Pb(NO3)2 and sodium formate in HEPES buffer. On transmission electron microscopy (TEM), the KR-12-formed PbNPs were spherical in shape and ranged from 3 to 8 nm. The PbNPs formed a line or curved pattern on bacteria. In addition, one or more pilus-like structures elongated from the bacteria. In contrast, Shewanella oneidensis MR-1 and other bacteria could not form PbNPs pattern or pilus-like structure under the same conditions. High-resolution TEM combined with energy-dispersive X-ray spectroscopy demonstrated that these PbNPs primarily contained Pb and had an amorphous structure. This is the first report of the biosynthesis of PbNPs by a Shewanella species.BacteriaBiosynthesisMicrobiologyPb Nanoparticles (PbNPs)Pilus-like structureShewanella[SDGs]SDG7journal article10.1007/s11051-015-3309-62-s2.0-84955452347WOS:000368731800002