2004-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/712253摘要：玉葉金花（Mussaenda pubescens）是台灣低海拔中常見的植物之一。玉葉金花常可以見到花萼其中的一個裂片尖端膨大成一個外形類似葉子、但顏色為白色或紅色的構造，稱為葉狀萼片（calycophylls, 或floral semaphylls）。而玉葉金花名稱的由來，即源於它的花序中黃色的花瓣和白色的葉狀萼片而來。這些葉狀萼片不管在細胞形狀或細胞排列上，都與正常的花瓣類似，加上有著顯著的顏色，一般皆認為這些葉狀萼片在功能上與花瓣無異。我們先前的假設是這些類花瓣的構造可能是由於原先控制花瓣的基因，在類花瓣構造的地方表現，而生成類似花瓣的構造。我們實驗室已經在玉葉金花中成功的鑑定出一個A群，三個B群，以及一個E群基因。由基因表現型式來看，其中有兩個B群基因在正常花萼和類花瓣花萼中都有表現，但是另一個B群基因卻沒有在各型花萼中表現。我們推測應該還有其他的因子參與其中，而很可能是與B群基因產物形成蛋白質複合體的未知基因。我們在此提出一個三年期的研究計畫，期望能自此獲得有關花瓣調控的各類因子。我們首先先利用在野生型和ABE基因突變的阿拉伯芥中大量表現玉葉金花ABE群基因的作法來檢測這些基因<br> Abstract: Mussaenda pubescens is a plant with enlarged and petaloid calyx lobe, named semaphylls. We are trying to elucidate the genetic mechanism for the formation of semaphylls. Current genetic ABCDE model suggests that floral organ identity is specified by combinations of the five classes of genes. According to the model and results from overexpression of A, B, and E class genes in Arabidopsis, it has been speculated that petaloid structure in plants might result from ectopic expression of petal identity genes. In our previous study on Mussaenda pubescens.we found that two of the three B class are expressed in semaphylls. We speculate that there are other factors involved in the semaphyll formation. We propose a three-year span experiments to identify the potential key factors responsible for the formation of petaloid structure. We propose to use yeast two-hybrid screening to identify putative counterparts of ABE genes since they form multimers or protein complex in other plants. We will first perform overexpression of M. pubescens ABE genes in wild type and ABE mutant background of Arabidopsis to examine their potential functions. We will perform yeast two-hybrid to determine the interactions among the ABE proteins identified from M. pubescens. In addition, we will perform yeast two-hybrid screening to identify proteins that interact with ABE gene products of M. pubescens. We will also conduct in vitro DNA binding assay for the ABE genes of M. pubescens. At last, we will perform RT-PCR and in situ hybridization for all the identified ABE genes and their interacting genes from M. pubescens. After the screening, hopefully we will be able to identify the genetic basis for petaloid structure in M. pubescens.玉葉金花花部器官決定基因蛋白質交互作用Mussaenda pubescensfloral organ identity genesprotein-protein interactions