2011-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/670669摘要：LsGRP1源自於葵百合，富含甘胺酸及半胱胺酸；水楊酸、撲殺熱處理及灰黴病菌感染時會增加百合葉片LsGRP1基因之表現。為充份了解LsGRP1在百合誘導抗病反應中所扮演的角色，以利LsGRP1的植保應用，擬研究LsGRP1之新發現功能-抗菌作用，包括抗菌活性及抗菌機制之探討。將利用大腸桿菌表現LsGRP1，用以測試LsGRP1對不同病原真菌及細菌的抑菌活性，在植體上觀察LsGRP1對目標真菌及細菌感染過程的影響，確認抑病的基礎。進一步以抗體偵測或螢光標定LsGRP1，利用螢光顯微鏡及電子顯微鏡觀察LsGRP1與目標真菌及細菌的結合情形及存在位置；加以比較LsGRP1處理前後之目標真菌及細菌的表面構造，並測試LsGRP1對細胞膜及胞內活性氧物質含量的影響，以推知LsGRP1的抗菌模式，供作LsGRP1應用發展的學理依據。<br> Abstract: Oriental lily ‘Star Gazer’ LsGRP1 protein contains many glycine and cysteine residues. LsGRP1 gene expression is increased in the leaves of lily plants after treatments of salicylic acid and probenazole, and Botrytis elliptica infection.In order to get better understanding on the roles of LsGRP1 in systemic resistance of lily to facilitate the application of LsGRP1 in plant protection, the new discovered function of LsGRP1 - antimicrobial activity will be studied,including antimicrobial assay and antimicrobial mechanism. LsGRP1 protein will be expressed by an Escherichia coli protein expression system and purified for antifungal and antibacterial assay, and the examination of infection process of target fungus and bacterium to confirm the disease control basis. Furthermore, by using antibody detection or fluorescence-labeled LsGRP1, the binding of LsGRP1 on the fungal and bacterial cells will be assayed by fluorescence microscopy and electron microscopy. The cell surface structure will be examined after LsGRP1 treatment. The effect of LsGRP1 on the cell membrane and the amount of reactive oxygen species in the cells will be investigated. These studies will facilitate the establishment of antimicrobial model of LsGRP1 which will become the basis of further application of this antimicrobial protein.抗菌胜&#32957抗菌分析病害防治Antimicrobial peptideAntimicrobial assayDisease Control