國立臺灣大學昆蟲學系暨研究所王重雄2006-07-252018-06-292006-07-252018-06-292002http://ntur.lib.ntu.edu.tw//handle/246246/12578蝦白點症(white spot syndrome)之病原 體稱之蝦白點症病毒(white spot syndrome virus, WSSV)。是危害蝦業養殖之重要病原 體。利用蝦血球細胞之初級培養以增殖蝦 白點症病毒的實驗已穫得初步的成功。並 經由電子顯微鏡的觀察證實此體外增殖法 確可用作蝦白點症病毒體外操作。從超微 構造及蝦白點症病毒形成團(virogenic stroma, VS)的分佈位置可知確有變異株存 在。VS 除了典型在核內外，胞質亦有病毒 形成團的存在。後者之胞核正常，但胞質 內之胞器多數已降解，而呈現許多空泡。 三種血球細胞中以透明血球(hyaline hematocytes)接受WSSV 感染為最高，其次 是半顆粒細胞 (semigranulocytes)，而顆粒 細胞 (granulocytes)最低。此結果在類淋巴 結(lymphoid organ)之電子顯微鏡的觀察下 亦證實有此二不同的病毒形成團存在，而 且在類淋巴結之各類細胞皆可發現。The causative agent of white spot syndrome (WSS) has been identified to be white spot syndrome virus (WSSV). WSSV is an important pathogen that causes a great impact on the shrimp culture industry. The in vitro propagation of WSSV in the primary culture of shrimp hemocyte had been established successfully in this study. Under electron microscopy, we not only confirmed our result, but also we found one WSSV mutant which formed a virogenic stroma in cytoplasm. The cytoplasm of infected cells with virogenic stroma showed the degenerated phenomena: degraded organelles and vacuolation, but their nuclei were more or less in normal shape. The hyaline hematocytes are the highest WSSV susceptibility cells, followed by semigranulocytes, and then granulocytes. The mutant was also confirmed by the electron microscopic observation of lymphoid organ and could be found in all kinds of lymphoid cells.application/pdf149527 bytesapplication/pdfzh-TW國立臺灣大學昆蟲學系暨研究所蝦白點症病毒蝦血球細胞變異株WSSVShrimp hemocytesmutantreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/12578/1/902611B002002.pdf